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CAND1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6168060

MBS6168060 is an antibody targeting CAND1. Also known as CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (APC). Host: Mouse. Mouse monoclonal antibody raised against a… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CAND1, Monoclonal Antibody
Full Product Name
CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (APC)
Product Gene Name
Product Synonym Names
Cullin-Associated and Neddylation-dissociated 1; DKFZp434M1414; FLJ10114; FLJ10929; FLJ38691; FLJ90441; KIAA0829; TIP120; TIP120A
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
5F2
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes CAND1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
136kDa
OMIM
607727
3D Structure
ModBase 3D Structure for Q86VP6
Immunogen
CAND1 (NP_060918, 1aa-100aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKE
Conjugate
APC

Product Categories/Family for anti-CAND1 antibody

Applications Tested/Suitable for anti-CAND1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CAND1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CAND1 antibody

CAND1 monoclonal antibody (M02), clone 5F2 Western Blot analysis of CAND1 expression in Hela S3 NE.
Mouse Anti-CAND1 Monoclonal Antibody – Cat# MBS6168060

Western Blot (WB) of anti-CAND1 antibody

CAND1 monoclonal antibody (M02), clone 5F2. Western Blot analysis of CAND1 expression in NIH/3T3.
Mouse Anti-CAND1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6168060

Western Blot (WB) of anti-CAND1 antibody

CAND1 monoclonal antibody (M02), clone 5F2. Western Blot analysis of CAND1 expression in PC-12.
Mouse Anti-CAND1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6168060

Western Blot (WB) of anti-CAND1 antibody

CAND1 monoclonal antibody (M02), clone 5F2. Western Blot analysis of CAND1 expression in Raw 264.7.
Mouse Anti-CAND1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6168060

Other Notes

Small volumes of anti-CAND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAND1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cullin-associated NEDD8-dissociated protein 1 isoform 1
NCBI Official Synonym Full Names
cullin associated and neddylation dissociated 1
NCBI Official Symbol
CAND1
NCBI Official Synonym Symbols
TIP120; TIP120A
UniProt Protein Name
Cullin-associated NEDD8-dissociated protein 1
UniProt Synonym Protein Names
Cullin-associated and neddylation-dissociated protein 1; TBP-interacting protein of 120 kDa A; TBP-interacting protein 120A; p120 CAND1
UniProt Gene Name
CAND1
UniProt Synonym Gene Names
KIAA0829; TIP120; TIP120A; TBP-interacting protein 120A
NCBI Protein Information
cullin-associated NEDD8-dissociated protein 1

NCBI Summary for CAND1

This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
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UniProt Comments for CAND1

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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