• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Developmental Pluripotency-Associated Protein 5 (DPPA5) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616988

DPPA5, also known as Esg-1 and ECAT2, is a 14kD RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid… More details

$1090 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Developmental Pluripotency-Associated Protein 5 (DPPA5), Polyclonal Antibody
Full Product Name
Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)
Product Gene Name
Product Synonym Names
Anti -Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Purity/Purification
Affinity Purified
Purified by mouse DPPA5 affinity chromatography and then biotinylated.
Specificity
Selected for use as a detection antibody in mouse DPPA5 Western Blots.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
13,498 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (74062787..74063953, complement). Location: 6q13
OMIM
611111
3D Structure
ModBase 3D Structure for A6NC42
Immunogen
E. coli-derived rmDPPA5

Product Categories/Family for anti-DPPA5 antibody

Applications Tested/Suitable for anti-DPPA5 antibody
Western Blot (WB)
Application Notes for anti-DPPA5 antibody
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and less than 10% cross-reactivity with rmDPPA4., Optimal dilutions should be determined by each laboratory for each application.

Other Notes

Small volumes of anti-DPPA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPPA5 purchase

MBS616988 is an antibody targeting DPPA5. Also known as Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin). Reactive in Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for DPPA5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
developmental pluripotency-associated 5 protein
NCBI Official Synonym Full Names
developmental pluripotency associated 5
NCBI Official Symbol
DPPA5
NCBI Official Synonym Symbols
ESG1
UniProt Protein Name
Developmental pluripotency-associated 5 protein
UniProt Synonym Protein Names
Embryonal stem cell-specific gene 1 protein
UniProt Gene Name
DPPA5
UniProt Synonym Gene Names
ESG1
UniProt Entry Name
DPPA5_HUMAN
NCBI Protein Information
developmental pluripotency-associated 5 protein; OTTHUMP00000016732; embryonal stem cell specific gene 1

NCBI Summary for DPPA5

This gene encodes a protein that may function in the control of cell pluripotency and early embryogenesis. Expression of this gene is a specific marker for pluripotent stem cells. Pseudogenes of this gene are located on the short arm of chromosome 10 and the long arm of chromosomes 14 and 19. [provided by RefSeq]
Show more

UniProt Comments for DPPA5

DPPA5: Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs. Belongs to the KHDC1 family.

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: cytoplasm

Molecular Function: RNA binding

Biological Process: multicellular organismal development
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-DPPA5 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000