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ATG3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618761

GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is… More details

$940 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Atg3, Polyclonal Antibody
Full Product Name
Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)
Product Gene Name
anti-ATG3 antibody
Product Synonym Names
Anti -Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Purity/Purification
Serum
Serum
Specificity
Recognizes human ATG3 at ~37kD.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
35,864 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (112251359..112280485, complement). Location: 3q13.2
OMIM
609606
3D Structure
ModBase 3D Structure for Q9NT62
Immunogen
Synthetic peptide corresponding to the central region of human ATG3, conjugated to Blue Carrier Protein. Cellular Localization: Cytoplasm. Species Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (canine).

Product Categories/Family for anti-ATG3 antibody

Antibodies; Abs to Autophagy
Applications Tested/Suitable for anti-ATG3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATG3 antibody
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry: 1:50-1:3000
Immunofluorescence: 1:50-1:3000
Western Blot: 1:50-1:3000

Testing Data of anti-ATG3 antibody

Confocal microscopy on IF detected ATG3 in cytospin-isolated human white blood cells using MBS618761, dilution of 1:100, incubated for 1h at RT. Cells stained for ATG3 appear red. Cells were counter stained with Hoechst Dye(blue colour). (Merged picture is presented). More info on staining:-Fixation:In acetone for 90 seconds. AB dilutions: MBS618761 diluted 1:100, incubated for 1h, washed 3x, and the secondary was incubated for 30min. Magnification 80X. No staining was observed using Pre-immunisation serum. No staining was observed in HL60 cell line using MBS618761
Rabbit Anti-ATG3 Polyclonal Antibody – Cat# MBS618761

Western Blot (WB) of anti-ATG3 antibody

Western Blot on mouse brain lysate using MBS618761 at a dilution of 1:50. Absorbption with the immunising peptide almost removed the three main bands otherwise visible on the unabsorbed strip.
Rabbit Anti-ATG3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS618761

Western Blot (WB) of anti-ATG3 antibody

Western Blot under reducing conditions on 293 cell lysate using MBS618761 at a dilution of 1:50.
Rabbit Anti-ATG3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS618761

Western Blot (WB) of anti-ATG3 antibody

Western Blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using MBS618761 at a dilution of 1:50.
Rabbit Anti-ATG3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS618761

Other Notes

Small volumes of anti-ATG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG3 purchase

MBS618761 is an antibody targeting ATG3. Also known as Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG3. It may not necessarily be applicable to this product.

NCBI GI #
119600077
NCBI GeneID
64422
UniProt Primary Accession #
Q9NT62
UniProt Secondary Accession #
Q6PKC5; Q9H6L9
UniProt Related Accession #
Q9NT62
NCBI Official Full Name
ATG3 autophagy related 3 homolog (S. cerevisiae), isoform CRA_d
NCBI Official Synonym Full Names
ATG3 autophagy related 3 homolog (S. cerevisiae)
NCBI Official Symbol
ATG3
NCBI Official Synonym Symbols
APG3; APG3L; PC3-96; FLJ22125; MGC15201; APG3-LIKE; DKFZp564M1178
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG3
UniProt Synonym Protein Names
Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96
Protein Family
Autophagy-related protein
UniProt Gene Name
ATG3
UniProt Synonym Gene Names
APG3; APG3L
UniProt Entry Name
ATG3_HUMAN
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG3; hApg3; 2610016C12Rik; OTTHUMP00000214547; OTTHUMP00000214548; autophagy-related protein 3

NCBI Summary for ATG3

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]

UniProt Comments for ATG3

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Autophagy; Enzyme, cellular metabolism

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: protein binding; enzyme binding; small conjugating protein ligase activity; APG8 conjugating enzyme activity; APG12 conjugating enzyme activity

Biological Process: mitochondrion degradation; protein ubiquitination; protein modification process; mitochondrial fragmentation during apoptosis; protein targeting to membrane; autophagic vacuole formation
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Research Articles on ATG3

1. These results unveil a role for ATG12-ATG3 in mitochondrial homeostasis and implicate the ATG12 conjugation system in cellular functions distinct from the early steps of autophagosome formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG3 antibody

 Reference Product  PubMed Publications
 ATG7 antibody  >22 publications with ATG3 and ATG7
 ATG12 antibody  >13 publications with ATG3 and ATG12
 GABARAP antibody  >6 publications with ATG3 and GABARAP
 GABARAPL2 antibody  >4 publications with ATG3 and GABARAPL2
 MAP1LC3B antibody  >2 publications with ATG3 and MAP1LC3B

Pathways associated with anti-ATG3 antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-ATG3 antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >2 publications with ATG3 and Neurodegenerative Diseases
 Anxiety Disorders Antibodies  >1 publications with ATG3 and Anxiety Disorders

Organs/Tissues associated with anti-ATG3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with ATG3 and Blood
 Vascular Antibodies  >1 publications with ATG3 and Vascular
 Kidney Antibodies  >1 publications with ATG3 and Kidney
 Eye Antibodies  >1 publications with ATG3 and Eye
 Ear Antibodies  >1 publications with ATG3 and Ear
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