Goat Anti-fto Polyclonal Antibody – Cat# MBS619275

EB08564 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

fto Antibody – Goat Polyclonal

Name: fto Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS619275
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619275

MBS619275 is an antibody targeting fto. Also known as FTO (Fatso, Fat Mass and Obesity-associated Protein, Protein Fatso). Reactive in Human. Host: Goat. More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FTO, Polyclonal Antibody

Full Product Name

FTO (Fatso, Fat Mass and Obesity-associated Protein, Protein Fatso)

Product Gene Name

anti-fto antibody

Product Synonym Names

Anti -FTO (Fatso, Fat Mass and Obesity-associated Protein, Protein Fatso)

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Purity/Purification

Affinity Purified
Purified by affinity chromatography.

Specificity

Recognizes FTO. Species Sequence Homology: human, rat, canine, porcine.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

58,527 Da

3D Structure

ModBase 3D Structure for Q68F54

Immunogen

Synthetic peptide corresponding to QQKPDCRPYWEKDD from the Internal region of FTO (NP_036066.2).

Product Categories/Family for anti-fto antibody

Antibodies; Abs to Obesity Proteins

Applications Tested/Suitable for anti-fto antibody

Western Blot (WB)

Application Notes for anti-fto antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:128,000

Other Notes

Small volumes of anti-fto antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for fto. It may not necessarily be applicable to this product.

NCBI GI #

148222349

NCBI GeneID

447305

NCBI Accession #

NP_001087481.1

UniProt Primary Accession #

Q68F54

UniProt Related Accession #

Q68F54

GenBank Nucleotide #

NM_001094012.1

NCBI Official Full Name

alpha-ketoglutarate-dependent dioxygenase FTO

NCBI Official Synonym Full Names

fat mass and obesity associated<

NCBI Official Symbol

fto

NCBI Official Synonym Symbols

MGC81641

UniProt Protein Name

Alpha-ketoglutarate-dependent dioxygenase FTO

Protein Family

Alpha-ketoglutarate-dependent dioxygenase

UniProt Gene Name

fto

UniProt Entry Name

FTO_XENLA

NCBI Protein Information

alpha-ketoglutarate-dependent dioxygenase FTO; protein fto

UniProt Comments for fto

Function: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. May contribute to the regulation of the global metabolic rate, energy expenditure and energy homeostasis

Cofactor: Binds 1 Fe2+ ion per subunit

Subcellular location: Nucleus

Domain: The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases

Sequence similarities: Belongs to the fto family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-fto antibody

Disease Name	Pubmed Publications
Obesity Antibodies	>326 publications with fto and Obesity
Neoplasms Antibodies	>47 publications with fto and Neoplasms
Insulin Resistance Antibodies	>34 publications with fto and Insulin Resistance
Liver Neoplasms Antibodies	>28 publications with fto and Liver Neoplasms
Liver Neoplasms, Experimental Antibodies	>25 publications with fto and Liver Neoplasms, Experimental
Weight Gain Antibodies	>22 publications with fto and Weight Gain
Inflammation Antibodies	>7 publications with fto and Inflammation
Carcinoma, Hepatocellular Antibodies	>4 publications with fto and Carcinoma, Hepatocellular
Adenocarcinoma Antibodies	>4 publications with fto and Adenocarcinoma
Colorectal Neoplasms Antibodies	>4 publications with fto and Colorectal Neoplasms

Organs/Tissues associated with anti-fto antibody

Organ/Tissue Name	Pubmed Publications
Whole Body Antibodies	>8 publications with fto and Whole Body