Mouse Anti-SPHK1 Monoclonal Antibody – Cat# MBS6192815

SPHK1 Antibody – Mouse Monoclonal

Name: SPHK1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6192815
Price: 960 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6192815

MaxLight405 is a new Violet photostable dye conjugate comparable to Alexa Fluor 405, PacificBlue, Brilliant Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (400nm); Emission (423nm); Extinction… More details

$960 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPHK1, Monoclonal Antibody

Full Product Name

SPHK1 (Sphingosine Kinase 1, SK 1, SPHK, SPK 1, SPK) (MaxLight 405)

Product Gene Name

anti-SPHK1 antibody

Product Synonym Names

EC=2.7.1.91

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

1D6

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

Purity/Purification

Purified by Protein A Affinity Chromatography.

Specificity

Recognizes human SPHK1.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

~71 kDa

Immunogen

Full length recombinant corresponding to aa1-385 from SPHK1 (AAH08040) with GST tag. MW of the GST tag alone is 26kD.

Immunogen Sequence

MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Conjugate

MaxLight405

Product Categories/Family for anti-SPHK1 antibody

Antibodies; Protein Kinases

Applications Tested/Suitable for anti-SPHK1 antibody

FLISA, Western Blot (WB)

Application Notes for anti-SPHK1 antibody

Applications are based on unconjugated antibody.

Product References and Citations for anti-SPHK1 antibody

1. The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen. Schroder M, Richter C, Juan MH, Maltusch K, Giegold O, Quintini G, Pfeilschifter JM, Huwiler A, Radeke HH.Mol Immunol. 2011 Mar 22. 2. The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line. Asher V, Warren A, Shaw R, Sowter H, Bali A, Khan R.Cancer Cell Int. 2011 Mar 10;11:6. 3. Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells. Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL.Neurochem Res. 2011 Jan 18. 4. Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9. Schenten V, Melchior C, Steinckwich N, Tschirhart EJ, Brechard S.J Leukoc Biol. 2011 Apr;89(4):587-96. Epub 2011 Jan 13. 5. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Sinha UK, Schorn VJ, Hochstim C, Chinn SB, Zhu S, Masood R.Wiley InterScience. DOI: 10.1002/ hed.21418 6. A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart. Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K.Glycobiology. 2010 Jan;20(4):473-84. Epub 2009 Dec 15. 7. G{alpha}q-mediated plasma membrane translocation of sphingosine kinase-1 and cross-activation of S1P receptors. Ter Braak M, Danneberg K, Lichte K, Liphardt K, Ktistakis NT, Pitson SM, Hla T, Jakobs KH, Heringdorf DM.Biochim Biophys Acta. 2009 Feb 2. 8. Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells. Billich A, Urtz N, Reuschel R, Baumruker T.Int J Biochem Cell Biol. 2009 Jul;41(7):1547-55. Epub 2009 Jan 8.

Other Notes

Small volumes of anti-SPHK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPHK1 purchase

MBS6192815 is an antibody targeting SPHK1. Also known as SPHK1 (Sphingosine Kinase 1, SK 1, SPHK, SPK 1, SPK) (MaxLight 405). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SPHK1. It may not necessarily be applicable to this product.

NCBI GI #

33872079

NCBI GeneID

8877

NCBI Accession #

BC008040

NCBI Related Accession #

AAH08040

UniProt Secondary Accession #

Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3

UniProt Related Accession #

Q9NYA1

NCBI Official Full Name

Homo sapiens sphingosine kinase 1, mRNA

NCBI Official Synonym Full Names

sphingosine kinase 1

NCBI Official Symbol

SPHK1

NCBI Official Synonym Symbols

SPHK

Protein Family

A-kinase anchor protein

NCBI Protein Information

sphingosine kinase 1

NCBI Summary for SPHK1

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Research Articles on SPHK1

1. our data suggest that SphK1 modulates the expression of EMT-related markers and cell migration by regulating the expression of p-FAK in CRC cells. Thus, SphK1 may play a functional role in mediating the EMT process in CRC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SPHK1 antibody

Products by Pathway	Pathway Diagram
Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies	Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
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Chaperonin-mediated Protein Folding Pathway antibodies	Chaperonin-mediated Protein Folding Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-SPHK1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>104 publications with SPHK1 and Neoplasms
Inflammation Antibodies	>52 publications with SPHK1 and Inflammation
Necrosis Antibodies	>32 publications with SPHK1 and Necrosis
Fibrosis Antibodies	>19 publications with SPHK1 and Fibrosis
Lung Diseases Antibodies	>18 publications with SPHK1 and Lung Diseases
Nervous System Diseases Antibodies	>17 publications with SPHK1 and Nervous System Diseases
Breast Neoplasms Antibodies	>14 publications with SPHK1 and Breast Neoplasms
Leukemia Antibodies	>14 publications with SPHK1 and Leukemia
Neoplasms, Experimental Antibodies	>11 publications with SPHK1 and Neoplasms, Experimental
Kidney Diseases Antibodies	>10 publications with SPHK1 and Kidney Diseases

Organs/Tissues associated with anti-SPHK1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>67 publications with SPHK1 and Blood
Vascular Antibodies	>42 publications with SPHK1 and Vascular
Lung Antibodies	>28 publications with SPHK1 and Lung
Brain Antibodies	>24 publications with SPHK1 and Brain
Kidney Antibodies	>23 publications with SPHK1 and Kidney
Bone Antibodies	>21 publications with SPHK1 and Bone
Heart Antibodies	>17 publications with SPHK1 and Heart
Prostate Antibodies	>15 publications with SPHK1 and Prostate
Bone Marrow Antibodies	>15 publications with SPHK1 and Bone Marrow
Liver Antibodies	>12 publications with SPHK1 and Liver