Sheep Anti-TLE3 Polyclonal Antibody – Cat# MBS619381

Transducin-Like Enhancer of Split 1 (TLE3) Antibody – Sheep Polyclonal

Name: TLE3 Antibody; Polyclonal; Sheep; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS619381
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619381

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Transducin-Like Enhancer of Split 1 (TLE3), Polyclonal Antibody

Full Product Name

Transducin-Like Enhancer of Split 1 (TLE1, E(Sp1) Homolog, Enhancer of Split Groucho-like Protein 1, ESG, ESG1, GRG1, Transducin-like Enhancer Protein 1)

Product Gene Name

anti-TLE3 antibody

Product Synonym Names

Anti -Transducin-Like Enhancer of Split 1 (TLE1, E(Sp1) Homolog, Enhancer of Split Groucho-like Protein 1, ESG, ESG1, GRG1, Transducin-like Enhancer Protein 1)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human

Form/Format

Supplied as a lyophillized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS.

Purity/Purification

Affinity Purified
Purified by affinity chromatography.

Specificity

Recognizes human TLE1. In direct ELISAs, this antibody shows ~10% cross-reactivity with rhTLE3 and rhTLE4 and less than 5% cross-reactivity with rhTLE2.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

83,417 Da

Chromosome Location

Chromosome: 15; NC_000015.9 (70340129..70390274, complement). Location: 15q22

OMIM

600190

3D Structure

ModBase 3D Structure for Q04726

Immunogen

E. coli-derived rhTLE1 (aa 11-200)

Product Categories/Family for anti-TLE3 antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-TLE3 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-TLE3 antibody

Dilution: ELISA: 0.5-1ug/ml. The detection limit for rhTLE1 is approximately 2ng/well.
Western Blot: 2ug/ml

Other Notes

Small volumes of anti-TLE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TLE3 purchase

MBS619381 is an antibody targeting TLE3. Also known as Transducin-Like Enhancer of Split 1 (TLE1, E(Sp1) Homolog, Enhancer of Split Groucho-like Protein 1, ESG, ESG1, GRG1, Transducin-like Enhancer Protein 1). Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for TLE3. It may not necessarily be applicable to this product.

NCBI GI #

46391800

NCBI GeneID

7090

NCBI Accession #

AAS90945.1

UniProt Primary Accession #

Q04726

UniProt Secondary Accession #

Q8IVV6; Q8WVR2; Q9HCM5; E9PD64

UniProt Related Accession #

Q04726

NCBI Official Full Name

transducin-like enhancer of split 3 splice variant 1

NCBI Official Synonym Full Names

transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)

NCBI Official Symbol

TLE3

NCBI Official Synonym Symbols

ESG; ESG3; GRG3; HsT18976

UniProt Protein Name

Transducin-like enhancer protein 3

UniProt Synonym Protein Names

Enhancer of split groucho-like protein 3

Protein Family

Transducin-like enhancer protein

UniProt Gene Name

TLE3

UniProt Synonym Gene Names

KIAA1547; ESG3

UniProt Entry Name

TLE3_HUMAN

NCBI Protein Information

transducin-like enhancer protein 3; enhancer of split groucho 3; enhancer of split groucho-like protein 3; transducin-like enhancer of split 3, homolog of Drosophila E(sp1)

NCBI Summary for TLE3

This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

UniProt Comments for TLE3

TLE3: transcriptional corepressor that binds to a number of transcription factors. Belongs to the WD-repeat Groucho/TLE family. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Four alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: Notch signaling pathway; organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; signal transduction

Research Articles on TLE3

1. High TLE3 expression predicts a favorable response to taxane containing chemotherapy regimens in ovarian carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TLE3 antibody

Reference Product	PubMed Publications
NKX2-2 antibody	>3 publications with TLE3 and NKX2-2
HDAC1 antibody	>1 publications with TLE3 and HDAC1
HESX1 antibody	>1 publications with TLE3 and HESX1

Pathways associated with anti-TLE3 antibody

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies	FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
NOTCH1 Intracellular Domain Regulates Transcription Pathway antibodies	NOTCH1 Intracellular Domain Regulates Transcription Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By NOTCH Pathway antibodies	Signaling By NOTCH Pathway Diagram
Signaling By NOTCH1 Pathway antibodies	Signaling By NOTCH1 Pathway Diagram
Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway antibodies	Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway Diagram
Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway antibodies	Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway Diagram
Signaling By NOTCH1 PEST Domain Mutants In Cancer Pathway antibodies	Signaling By NOTCH1 PEST Domain Mutants In Cancer Pathway Diagram
Signaling By NOTCH1 In Cancer Pathway antibodies	Signaling By NOTCH1 In Cancer Pathway Diagram

Diseases associated with anti-TLE3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>10 publications with TLE3 and Neoplasms
Breast Neoplasms Antibodies	>5 publications with TLE3 and Breast Neoplasms
Nervous System Diseases Antibodies	>2 publications with TLE3 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>1 publications with TLE3 and Neoplasms, Experimental
Skin Neoplasms Antibodies	>1 publications with TLE3 and Skin Neoplasms
Uterine Cervical Neoplasms Antibodies	>1 publications with TLE3 and Uterine Cervical Neoplasms
Kidney Neoplasms Antibodies	>1 publications with TLE3 and Kidney Neoplasms
Brain Diseases Antibodies	>1 publications with TLE3 and Brain Diseases
Hyperplasia Antibodies	>1 publications with TLE3 and Hyperplasia
Neoplasm Metastasis Antibodies	>1 publications with TLE3 and Neoplasm Metastasis

Organs/Tissues associated with anti-TLE3 antibody

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>4 publications with TLE3 and Pancreas
Brain Antibodies	>4 publications with TLE3 and Brain
Blood Antibodies	>3 publications with TLE3 and Blood
Placenta Antibodies	>3 publications with TLE3 and Placenta
Embryonic Tissue Antibodies	>3 publications with TLE3 and Embryonic Tissue
Vascular Antibodies	>2 publications with TLE3 and Vascular
Connective Tissue Antibodies	>1 publications with TLE3 and Connective Tissue
Cervix Antibodies	>1 publications with TLE3 and Cervix
Lymph Node Antibodies	>1 publications with TLE3 and Lymph Node
Kidney Antibodies	>1 publications with TLE3 and Kidney