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Smad1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620315

SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate… More details

$1140 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Smad1, Polyclonal Antibody
Full Product Name
Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Product Synonym Names
Anti -Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human. Species Sequence Homology: duckbill platypus, drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
~52kD
Immunogen
Synthetic peptide corresponding to the region of aa containing serine 206 of human SMAD1 protein.
Applications Tested/Suitable for anti-Smad1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Smad1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:25,000-1:30,000
Western Blot: 1:500

Western Blot (WB) of anti-Smad1 antibody

Western blot using Immunochemicals Affinity Purified Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 _g of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n 600-401-886) staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL.
Rabbit Anti-Smad1 Polyclonal Antibody – Cat# MBS620315

Other Notes

Small volumes of anti-Smad1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smad1 purchase

MBS620315 is an antibody targeting Smad1. Also known as Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Smad1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
SMAD1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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