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CTP Phosphocholine Cytidylyltransferase (PCYT1A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620699

CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTa, CCTB2, and CCTB3 (1,2). CCTa is the major… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CTP Phosphocholine cytidylyltransferase (PCYT1A), Polyclonal Antibody
Full Product Name
CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa)
Product Gene Name
Product Synonym Names
Anti -CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CTP Phosphocholine Cytidylytransferase at 42kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
41,731 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (195965253..196014584, complement). Location: 3q29
OMIM
123695
3D Structure
ModBase 3D Structure for P49585
Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human CCT Alpha protein.

Product Categories/Family for anti-PCYT1A antibody

Applications Tested/Suitable for anti-PCYT1A antibody
Western Blot (WB), Immunoprecipitation (IP). Other applications not tested.
Application Notes for anti-PCYT1A antibody
Suitable for use in Western Blot and Immunoprecipitation.
Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-PCYT1A antibody

Western Blot analysis of extracts from HT-1080 and K-562 cells using MBS620699.
Rabbit Anti-PCYT1A Polyclonal Antibody – Cat# MBS620699

Other Notes

Small volumes of anti-PCYT1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCYT1A purchase

MBS620699 is an antibody targeting PCYT1A. Also known as CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCYT1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CTP:phosphocholine cytidylyltransferase
NCBI Official Synonym Full Names
phosphate cytidylyltransferase 1, choline, alpha
NCBI Official Symbol
PCYT1A
NCBI Official Synonym Symbols
CT; CTA; CCTA; CTPCT; PCYT1
UniProt Protein Name
Choline-phosphate cytidylyltransferase A
UniProt Synonym Protein Names
CCT-alpha; CTP:phosphocholine cytidylyltransferase A; CCT A; CT A; Phosphorylcholine transferase A
UniProt Gene Name
PCYT1A
UniProt Synonym Gene Names
CTPCT; PCYT1
UniProt Entry Name
PCY1A_HUMAN
NCBI Protein Information
choline-phosphate cytidylyltransferase A; CT A; CCT A; CCT-alpha; OTTHUMP00000208442; OTTHUMP00000208443; OTTHUMP00000208444; OTTHUMP00000208447; phosphorylcholine transferase A; CTP:phosphocholine cytidylyltransferase A

NCBI Summary for PCYT1A

This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]
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UniProt Comments for PCYT1A

PCYT1A: Controls phosphatidylcholine synthesis. Homodimer. By phosphorylation. Belongs to the cytidylyltransferase family.

Protein type: Transferase; EC 2.7.7.15; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; glycogen granule; cytosol

Molecular Function: choline-phosphate cytidylyltransferase activity; protein homodimerization activity; lipid binding

Biological Process: CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process

Disease: Spondylometaphyseal Dysplasia With Cone-rod Dystrophy
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PCYT1A antibody

 Reference Product  PubMed Publications
 ACTB antibody  >1 publications with PCYT1A and ACTB
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