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Occludin (OCLN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS621431

MBS621431 is an antibody targeting OCLN. Also known as Occludin. Reactive in Canine, Human, Mammal, Mouse, Rat. Host: Rabbit. More details

$910 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Occludin (OCLN), Polyclonal Antibody
Full Product Name
Occludin
Product Gene Name
anti-OCLN antibody
Product Synonym Names
Anti -Occludin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Canine, Human, Mammal, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species Crossreactivity: mammalian, mouse, rat and canine
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
59,144 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (68788119..68850397). Location: 5q13.1
OMIM
251290
3D Structure
ModBase 3D Structure for Q16625
Immunogen
Fusion protein containing the C-terminal 150aa of human occludin.

Product Categories/Family for anti-OCLN antibody

Antibodies; Abs to Signal Transduction
Applications Tested/Suitable for anti-OCLN antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-OCLN antibody
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunofluorescence: 4-20ug/ml
Immunoprecipitation: ~5ug/IP reaction
Immunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37 degree C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody.

Western Blot (WB) of anti-OCLN antibody

Western Blot analysis of MDCK cels using MBS621431
Rabbit Anti-OCLN Polyclonal Antibody – Cat# MBS621431

Immunohistochemistry (IHC) of anti-OCLN antibody

Immunohistochemical staining of breast carcinoma using MBS621431
Rabbit Anti-OCLN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS621431

Immunofluorescence (IF) of anti-OCLN antibody

Immunofluorescent staining of human occludin-transfected rat fibroblasts using MBS621431
Rabbit Anti-OCLN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS621431

Immunofluorescence (IF) of anti-OCLN antibody

Immunofluorescent staining of MDCK canine cells using MBS621431
Rabbit Anti-OCLN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS621431

Other Notes

Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #
4505487
NCBI GeneID
100506658
NCBI Accession #
NP_002529.1
UniProt Primary Accession #
Q16625
UniProt Secondary Accession #
Q8N6K1; B5BU70
UniProt Related Accession #
Q16625; Q5U1V4
GenBank Nucleotide #
NM_002538.3
NCBI Official Full Name
occludin isoform a
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
OCLN
NCBI Official Synonym Symbols
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
OCLN
UniProt Entry Name
OCLN_HUMAN
NCBI Protein Information
occludin; OTTHUMP00000125187; OTTHUMP00000222347; tight junction protein occludin

NCBI Summary for OCLN

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq]
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UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
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Research Articles on OCLN

1. occludin S408 dephosphorylation regulates paracellular permeability by remodeling tight junction protein dynamic behavior and intermolecular interactions between occludin, ZO-1, and select claudinszo

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OCLN antibody

 Reference Product  PubMed Publications
 TJP1 antibody  >3 publications with OCLN and TJP1

Pathways associated with anti-OCLN antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram

Diseases associated with anti-OCLN antibody

 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >9 publications with OCLN and Hepatitis
 Liver Diseases Antibodies  >5 publications with OCLN and Liver Diseases
 Neoplasms Antibodies  >5 publications with OCLN and Neoplasms
 Liver Neoplasms Antibodies  >4 publications with OCLN and Liver Neoplasms
 Leukemia Antibodies  >3 publications with OCLN and Leukemia
 Central Nervous System Diseases Antibodies  >3 publications with OCLN and Central Nervous System Diseases
 Cardiovascular Diseases Antibodies  >2 publications with OCLN and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with OCLN and Carcinoma, Hepatocellular
 Nervous System Malformations Antibodies  >2 publications with OCLN and Nervous System Malformations
 Vascular Diseases Antibodies  >2 publications with OCLN and Vascular Diseases

Organs/Tissues associated with anti-OCLN antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with OCLN and Testis
 Lung Antibodies  >1 publications with OCLN and Lung
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