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Collectrin (TMEM27) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS622578

Collectrin (also Transmembrane protein 27) is a 37-43kD member of the MEM27 family of proteins. It is expressed on pancreatic beta-cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse… More details

$1165 / 0.05 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Collectrin (TMEM27), Polyclonal Antibody
Full Product Name
Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679, PRO1312) (Biotin)
Product Gene Name
Product Synonym Names
Anti -Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679, PRO1312) (Biotin)
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Mouse
Form/Format
Supplied as lyophilized powder from PBS, 2.5mg BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris-buffered saline, pH 7.3 (20mM Tris base, 150mM sodium chloride), 0.1% BSA.
Purity/Purification
Affinity Purified
Purified by Immunoaffinity chromatography
Specificity
Recognizes the extracellular domain of mouse Collectrin. Shows ~40% crossreactivity with recombinant human Collectrin.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months. Reconstitute with sterile Tris-buffered saline, pH 7.3 (20mM Tris base, 150mM sodium chloride), 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
25,235 Da
Chromosome Location
Chromosome: X; NC_000023.10 (15645441..15683154, complement). Location: Xp22
OMIM
300631
3D Structure
ModBase 3D Structure for Q9HBJ8
Immunogen
Purified, recombinant protein corresponding to aa15-144 of mouse Collectrin extracellular domain, expressed in NSO cells (Q9ESG4).
Species Sequence Homology
Rat; 92%, human; 86%
Applications Tested/Suitable for anti-TMEM27 antibody
Western Blot (WB)
Application Notes for anti-TMEM27 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse Collectrin is ~1ng/lane under non-reducing and reducing conditions.

Other Notes

Small volumes of anti-TMEM27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TMEM27 purchase

MBS622578 is an antibody targeting TMEM27. Also known as Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679, PRO1312) (Biotin). Reactive in Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for TMEM27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collectrin
NCBI Official Synonym Full Names
transmembrane protein 27
NCBI Official Symbol
TMEM27
NCBI Official Synonym Symbols
NX17; NX-17
UniProt Protein Name
Collectrin
UniProt Synonym Protein Names
Transmembrane protein 27
Protein Family
UniProt Gene Name
TMEM27
UniProt Entry Name
TMM27_HUMAN
NCBI Protein Information
collectrin; 0610008J07Rik; OTTHUMP00000022967; kidney-specific membrane protein

NCBI Summary for TMEM27

This gene encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. [provided by RefSeq]
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UniProt Comments for TMEM27

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: brush border membrane; cytoplasm; integral to membrane

Molecular Function: peptidyl-dipeptidase activity; protein binding; metallopeptidase activity

Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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