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STAT3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623970

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as… More details

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Product Overview

Online Datasheet in PDF
Product Name
STAT3, Polyclonal Antibody
Full Product Name
STAT3, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)
Product Gene Name
anti-Stat3 antibody
Product Synonym Names
Anti -STAT3, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human STAT3 when phosphorylated at Ser727.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
88,054 Da
Chromosome Location
Chromosome: 11; NC_000077.5 (100748124..100800825, complement). Location: 11 D; 11 60.5 cM
3D Structure
ModBase 3D Structure for P42227
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser727 of human STAT3 (KLH).

Product Categories/Family for anti-Stat3 antibody

Antibodies; Abs to Transcription Factors; STAT
Applications Tested/Suitable for anti-Stat3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Stat3 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Immunofluorescence (IF) of anti-Stat3 antibody

Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3260a phospho-STAT3-S727 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data .
Rabbit Anti-STAT3 Polyclonal Antibody – Cat# MBS623970

Western Blot (WB) of anti-Stat3 antibody

The Phospho-STAT3-S727 Pab is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
Rabbit Anti-STAT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS623970

Western Blot (WB) of anti-Stat3 antibody

The phospho-STAT3-Ser727 Pab is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.
Rabbit Anti-STAT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS623970

Immunohistochemistry (IHC) of anti-Stat3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-STAT3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS623970

Other Notes

Small volumes of anti-Stat3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Stat3 purchase

MBS623970 is an antibody targeting Stat3. Also known as STAT3, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Stat3. It may not necessarily be applicable to this product.

NCBI GI #
458706
NCBI GeneID
20848
UniProt Primary Accession #
P42227
UniProt Secondary Accession #
A2A5D1; B7ZC17
UniProt Related Accession #
P42227; Q3U5Q4; Q3U6S9; Q3ULI4; Q6GU23; Q8CFJ6; Q99ML3
NCBI Official Full Name
Stat3
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3
NCBI Official Symbol
Stat3
NCBI Official Synonym Symbols
Aprf; AW109958; 1110034C02Rik
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Synonym Protein Names
Acute-phase response factor
Protein Family
Signal transducer and activator of transcription
UniProt Gene Name
Stat3
UniProt Synonym Gene Names
Aprf
UniProt Entry Name
STAT3_MOUSE
NCBI Protein Information
signal transducer and activator of transcription 3; OTTMUSP00000002052; OTTMUSP00000002053; OTTMUSP00000045315; OTTMUSP00000045316; acute phase response factor; acute-phase response factor

UniProt Comments for Stat3

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; cytoplasm; plasma membrane; intracellular; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; ligand-dependent nuclear receptor activity; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; protein phosphatase binding; protein kinase binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation
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Research Articles on Stat3

1. butein exerts its antiproliferative and proapoptotic effects through suppression of STAT3 signaling in HCC both in vitro and in vivo

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Stat3 antibody

 Reference Product  PubMed Publications
 Src antibody  >574 publications with Stat3 and Src
 Jak1 antibody  >448 publications with Stat3 and Jak1
 Il6st antibody  >247 publications with Stat3 and Il6st
 Jun antibody  >241 publications with Stat3 and Jun
 Map3k1 antibody  >10 publications with Stat3 and Map3k1
 Prkcd antibody  >7 publications with Stat3 and Prkcd
 Pik3r1 antibody  >2 publications with Stat3 and Pik3r1

Pathways associated with anti-Stat3 antibody

 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram

Diseases associated with anti-Stat3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2396 publications with Stat3 and Neoplasms
 Inflammation Antibodies  >906 publications with Stat3 and Inflammation
 Carcinoma Antibodies  >854 publications with Stat3 and Carcinoma
 Leukemia Antibodies  >849 publications with Stat3 and Leukemia
 Cardiovascular Diseases Antibodies  >512 publications with Stat3 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >371 publications with Stat3 and Adenocarcinoma
 Liver Diseases Antibodies  >355 publications with Stat3 and Liver Diseases
 Neoplasms, Experimental Antibodies  >269 publications with Stat3 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >266 publications with Stat3 and Breast Neoplasms
 Lymphoma Antibodies  >247 publications with Stat3 and Lymphoma

Organs/Tissues associated with anti-Stat3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1639 publications with Stat3 and Blood
 Other Antibodies  >1119 publications with Stat3 and Other
 Liver Antibodies  >812 publications with Stat3 and Liver
 Brain Antibodies  >636 publications with Stat3 and Brain
 Muscle Antibodies  >485 publications with Stat3 and Muscle
 Bone Antibodies  >462 publications with Stat3 and Bone
 Lung Antibodies  >420 publications with Stat3 and Lung
 Heart Antibodies  >343 publications with Stat3 and Heart
 Bone Marrow Antibodies  >316 publications with Stat3 and Bone Marrow
 Skin Antibodies  >278 publications with Stat3 and Skin
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