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Fibroblast Activation Protein Alpha (FAPa) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2030833
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2030833 is a recombinant protein targeting FAPa. Also known as Recombinant Fibroblast Activation Protein Alpha (FAPa). Host: E ColiSource: Prokaryotic expression. More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Activation Protein Alpha (FAPa), Recombinant Protein
Full Product Name
Recombinant Fibroblast Activation Protein Alpha (FAPa)
Product Gene Name
FAPa recombinant protein
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
GGPCSQSVK SVFAVNWITY LASKEGIVIA LVDGRGTAFQ GDKFLHAVYR KLGVYEVEDQ LTAVRKFIEM GFIDEERIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV SSWEYYASIY SERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT ADDNVHFQNS AQIAKALVNA QVDFQAMWYS DQNHGISSGR SQNHLYTHMT HFLKQCFSLS D
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Purity/Purification
>95%
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: FAPa (MBS2026729)
Immunogen: FAPa (MBS2030833)
Matching Pairs
APC-CY7 Conjugated Antibody: FAPa (MBS2065901)
Immunogen: FAPa (MBS2030833)
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Molecular Weight
26,954 Da
OMIM
600403
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Predicted Molecular Mass
28.4kDa
Accurate Molecular Mass (KD)
28kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Mus musculus (Mouse)
Fragment
Gly542~Asp761
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for FAPa recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of FAPa recombinant protein

FAPa Recombinant Protein – Cat# MBS2030833

SDS-Page of FAPa recombinant protein

FAPa Recombinant Protein, SDS-Page – Cat# MBS2030833

Testing Data of FAPa recombinant protein

FAPa Recombinant Protein, Testing Data – Cat# MBS2030833

Other Notes

Small volumes of FAPa recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FAPa. It may not necessarily be applicable to this product.

NCBI GI #
119631758
NCBI GeneID
2191
NCBI Accession #
EAX11353.1
UniProt Secondary Accession #
O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
UniProt Related Accession #
Q12884
NCBI Official Full Name
fibroblast activation protein, alpha, isoform CRA_a
NCBI Official Synonym Full Names
fibroblast activation protein, alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; SIMP; DPPIV
UniProt Protein Name
Prolyl endopeptidase FAP
UniProt Synonym Protein Names
170 kDa melanoma membrane-bound gelatinase
Protein Family
Prolyl endopeptidase
UniProt Gene Name
FAP
UniProt Synonym Gene Names
APCE
UniProt Entry Name
SEPR_HUMAN
NCBI Protein Information
prolyl endopeptidase FAP

NCBI Summary for FAPa

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for FAPa

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.14.5; Membrane protein, integral; EC 3.4.21.26

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; focal adhesion; cell surface; apical part of cell; lamellipodium; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein dimerization activity; peptidase activity; integrin binding; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; cell adhesion; proteolysis; regulation of fibrinolysis
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Research Articles on FAPa

1. High FAP expression is associated with increased metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FAPa recombinant protein

 Reference Product  PubMed Publications
 DPP4 recombinant protein  >19 publications with FAPa and DPP4
 PREP recombinant protein  >5 publications with FAPa and PREP
 DPP7 recombinant protein  >3 publications with FAPa and DPP7
 NPY recombinant protein  >2 publications with FAPa and NPY
 PYY recombinant protein  >2 publications with FAPa and PYY
 GIP recombinant protein  >2 publications with FAPa and GIP

Diseases associated with FAPa recombinant protein

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >617 publications with FAPa and Carcinoma
 Adenocarcinoma Antibodies  >288 publications with FAPa and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >170 publications with FAPa and Cardiovascular Diseases
 Hypertrophy Antibodies  >103 publications with FAPa and Hypertrophy
 Inflammation Antibodies  >103 publications with FAPa and Inflammation
 Disease Models, Animal Antibodies  >96 publications with FAPa and Disease Models, Animal
 Breast Neoplasms Antibodies  >75 publications with FAPa and Breast Neoplasms
 Thyroid Diseases Antibodies  >73 publications with FAPa and Thyroid Diseases
 Necrosis Antibodies  >47 publications with FAPa and Necrosis
 Fibrosis Antibodies  >43 publications with FAPa and Fibrosis

Organs/Tissues associated with FAPa recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >450 publications with FAPa and Liver
 Intestine Antibodies  >429 publications with FAPa and Intestine
 Vascular Antibodies  >185 publications with FAPa and Vascular
 Muscle Antibodies  >178 publications with FAPa and Muscle
 Stomach Antibodies  >151 publications with FAPa and Stomach
 Eye Antibodies  >141 publications with FAPa and Eye
 Brain Antibodies  >126 publications with FAPa and Brain
 Thyroid Antibodies  >116 publications with FAPa and Thyroid
 Skin Antibodies  >107 publications with FAPa and Skin
 Lung Antibodies  >99 publications with FAPa and Lung
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