Mouse Anti-CD146 Monoclonal Antibody – Cat# MBS6242159

HUVEC lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C2548-78.

CD146 Antibody – Mouse Monoclonal

Name: CD146 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS6242159
Price: 1245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6242159

MBS6242159 is an antibody targeting CD146. Also known as CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM) (APC). Reactive in Canine, Human, Mouse, Rabbit. Host:… More details

$1245 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD146, Monoclonal Antibody

Full Product Name

CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM) (APC)

Product Gene Name

anti-CD146 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

2Q401

Host

Mouse

Species Reactivity

Canine, Human, Mouse, Rabbit

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Protein A Affinity Chromatography

Specificity

Recognizes human CD146, Mr 110kD. In blood and marrow, reacts only with endothelial cells. Species Crossreactivity: canine, rabbit and mouse (Detects circulating endothelial cells).

Preparation and Storage

Store at 4 degree C. Do Not Freeze.

Molecular Weight

61.0kDa (547aa) 70-100KDa (SDS-PAGE under reducing conditions.)

OMIM

155735

3D Structure

ModBase 3D Structure for P43121

Immunogen

HUVEC (Accession #NP_006491)

Conjugate

APC

Product Categories/Family for anti-CD146 antibody

Antibodies; CD Markers

Applications Tested/Suitable for anti-CD146 antibody

Flow Cytometry (FC/FACS), FLISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product References and Citations for anti-CD146 antibody

1. Solovey, A., et al., New England Journal of Medicine 337: 1584 (1997). 2. Gui, et al., Blood Supplement Abstract #697 (1992). 3. St. Croix, et al., Science 289: 1197-1202 (2000).

Other Notes

Small volumes of anti-CD146 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD146. It may not necessarily be applicable to this product.

NCBI GI #

71274107

NCBI GeneID

4162

NCBI Accession #

NP_006491

UniProt Primary Accession #

P43121

UniProt Secondary Accession #

O95812; Q59E86; Q6PHR3; Q6ZTR2

UniProt Related Accession #

P43121

GenBank Nucleotide #

NM_006500.2

NCBI Official Full Name

cell surface glycoprotein MUC18

NCBI Official Synonym Full Names

melanoma cell adhesion molecule

NCBI Official Symbol

MCAM

NCBI Official Synonym Symbols

CD146; MUC18

UniProt Protein Name

Cell surface glycoprotein MUC18

UniProt Synonym Protein Names

Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146

Protein Family

Cell surface glycoprotein

UniProt Gene Name

MCAM

UniProt Synonym Gene Names

MUC18

UniProt Entry Name

MUC18_HUMAN

NCBI Protein Information

cell surface glycoprotein MUC18

UniProt Comments for CD146

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration

Research Articles on CD146

1. We demonstrate that ER(+) breast cancers contain two CAF subtypes defined by CD146 expression. CD146(neg) CAFs suppress ER expression in ER(+) breast cancer cells, decrease tumor cell sensitivity to estrogen, and increase tumor cell resistance to tamoxifen therapy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD146 antibody

Reference Product	PubMed Publications
FGF2 antibody	>8 publications with CD146 and FGF2
PTK2 antibody	>4 publications with CD146 and PTK2
TGFB1 antibody	>4 publications with CD146 and TGFB1
ATF3 antibody	>2 publications with CD146 and ATF3
LGALS1 antibody	>2 publications with CD146 and LGALS1
ESR1 antibody	>2 publications with CD146 and ESR1
CYP1B1 antibody	>1 publications with CD146 and CYP1B1
EDN1 antibody	>1 publications with CD146 and EDN1

Diseases associated with anti-CD146 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>41 publications with CD146 and Inflammation
Lung Diseases Antibodies	>32 publications with CD146 and Lung Diseases
Disease Models, Animal Antibodies	>29 publications with CD146 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>26 publications with CD146 and Neoplasms, Experimental
Cardiovascular Diseases Antibodies	>26 publications with CD146 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>25 publications with CD146 and Adenocarcinoma
Lung Neoplasms Antibodies	>24 publications with CD146 and Lung Neoplasms
Breast Neoplasms Antibodies	>23 publications with CD146 and Breast Neoplasms
Nervous System Diseases Antibodies	>20 publications with CD146 and Nervous System Diseases
Prostatic Neoplasms Antibodies	>17 publications with CD146 and Prostatic Neoplasms

Organs/Tissues associated with anti-CD146 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>148 publications with CD146 and Blood
Vascular Antibodies	>115 publications with CD146 and Vascular
Skin Antibodies	>55 publications with CD146 and Skin
Bone Antibodies	>55 publications with CD146 and Bone
Lung Antibodies	>49 publications with CD146 and Lung
Embryonic Tissue Antibodies	>41 publications with CD146 and Embryonic Tissue
Bone Marrow Antibodies	>38 publications with CD146 and Bone Marrow
Muscle Antibodies	>31 publications with CD146 and Muscle
Brain Antibodies	>25 publications with CD146 and Brain
Liver Antibodies	>23 publications with CD146 and Liver