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Histone H3 (HIST3H3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6243008

MBS6243008 is an antibody targeting HIST3H3. Also known as Histone H3, monomethyl (Lys9) (Biotin). Reactive in Human. Host: Mouse. More details

$1450 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3 (HIST3H3), Monoclonal Antibody
Full Product Name
Histone H3, monomethyl (Lys9) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10A19
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
15,508 Da
OMIM
300254
3D Structure
ModBase 3D Structure for O43463
Immunogen
Linear peptide corresponding to monomethylated Lys9 at the N-terminal region of human Histone H3, conjugated to KLH.
Conjugate
Biotin

Product Categories/Family for anti-HIST3H3 antibody

Applications Tested/Suitable for anti-HIST3H3 antibody
Western Blot (WB)

Western Blot (WB) of anti-HIST3H3 antibody

Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Mouse Anti-HIST3H3 Monoclonal Antibody – Cat# MBS6243008

Western Blot (WB) of anti-HIST3H3 antibody

Western Blot: Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS608628 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Mouse Anti-HIST3H3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6243008

Product References and Citations for anti-HIST3H3 antibody

1. Nakayama, J.I., et al., Science 292: 110-113 (2001).

Other Notes

Small volumes of anti-HIST3H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST3H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
UniProt Protein Name
Histone-lysine N-methyltransferase SUV39H1
UniProt Synonym Protein Names
Histone H3-K9 methyltransferase 1; H3-K9-HMTase 1; Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1
Protein Family
UniProt Gene Name
SUV39H1
UniProt Synonym Gene Names
KMT1A; SUV39H; H3-K9-HMTase 1
UniProt Entry Name
SUV91_HUMAN
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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