• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Catenin, Beta, Non-Phosphorylated Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627325

B-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates B-catenin on Ser45. This phosphorylation… More details

$830 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Catenin, beta, non-phosphorylated, Polyclonal Antibody
Full Product Name
Catenin, beta, non-phosphorylated (Ser33/37/Thr41)
Product Synonym Names
Anti -Catenin, beta, non-phosphorylated (Ser33/37/Thr41)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied in 10 mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human non-phospho-B-Catenin (Ser33/37/Thr41). Species crossreactivity: rat
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a region surrounding residue Ser37 of human B-catenin (blue carrier-coupled).
Applications Tested/Suitable for anti-Catenin, beta, non-phosphorylated antibody
Western Blot (WB)
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.

Western Blot (WB) of anti-Catenin, beta, non-phosphorylated antibody

Western blot analysis of SW480 cells treated with Calyculin A and immunoprecipitated using Phospho-β-Catenin (Ser33/37) antibody (Lane 2), β-Catenin Antibody (Amino-terminal Antigen) (Lane 3) and an unrelated rabbit IgG as negative control (Lane 4). The immunoprecipitates along with the input lysate (Lane 1) were subject to western analysis using β-Catenin mAb (upper), Phospho-β-Catenin (Ser33/37) antibody (middle), and Non-phospho-β-catenin (Ser33/37/Thr41) antibody (lower).
Rabbit Anti-Catenin, Beta, Non-Phosphorylated Polyclonal Antibody – Cat# MBS627325

Western Blot (WB) of anti-Catenin, beta, non-phosphorylated antibody

Western blot analysis of total SW480 cell lysates using Non-phospho-β-Catenin (Ser33/Ser37/Thr41) antibody in the presence of a non-phospho-β-catenin peptide (Lane 4) and various peptides phosphorylated at different positions, including phospho-β-catenin (Ser33) peptide (Lane 1), phospho-β-catenin (Ser33/37) peptide (Lane 2), phospho-β-catenin (Ser33/37/Thr41) peptide (Lane 3), phospho-β-catenin (Ser37) peptide (Lane 5), phospho-β-catenin (Ser37/Thr41) peptide (Lane 6), and phospho-β-catenin (Thr41) peptide (Lane 7).
Rabbit Anti-Catenin, Beta, Non-Phosphorylated Polyclonal Antibody, Western Blot (WB) – Cat# MBS627325

Other Notes

Small volumes of anti-Catenin, beta, non-phosphorylated antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Catenin, beta, non-phosphorylated purchase

MBS627325 is an antibody targeting Catenin, beta, non-phosphorylated. Also known as Catenin, beta, non-phosphorylated (Ser33/37/Thr41). Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Catenin, beta, non-phosphorylated. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
Chain C, Mouse Importin Alpha: Non-Phosphorylated Sv40 Cn Peptide Complex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000