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ARL8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6275194

ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARL8, Polyclonal Antibody
Full Product Name
ARL8, CT (ARL5B, ARL8, ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8) (APC)
Product Gene Name
Product Synonym Gene Name
ARL5B
Product Synonym Names
ARL5B; ARL8; ADP-ribosylation factor-like protein 5B; ADP-ribosylation factor-like protein 8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
20,375 Da
OMIM
608909
3D Structure
ModBase 3D Structure for Q96KC2
Immunogen
ARL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-161 amino acids from the C-terminal region of human ARL8.
Conjugate
APC
Note
Preservative Free

Product Categories/Family for anti-ARL8 antibody

Applications Tested/Suitable for anti-ARL8 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ARL8 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ARL8 antibody

ARL8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARL8 antibody detected the ARL8 protein (arrow).
Rabbit Anti-ARL8 Polyclonal Antibody – Cat# MBS6275194

Immunohistochemistry (IHC) of anti-ARL8 antibody

ARL8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ARL8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6275194

Flow Cytometry (FC/FACS) of anti-ARL8 antibody

ARL8 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ARL8 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6275194

Product References and Citations for anti-ARL8 antibody

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Sebald, E., et al. Gene 311, 147-151 (2003).

Other Notes

Small volumes of anti-ARL8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARL8 purchase

MBS6275194 is an antibody targeting ARL8. Also known as ARL8, CT (ARL5B, ARL8, ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARL8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ADP-ribosylation factor-like protein 5B
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 5B
NCBI Official Symbol
ARL5B
NCBI Official Synonym Symbols
ARL8
UniProt Protein Name
ADP-ribosylation factor-like protein 5B
UniProt Synonym Protein Names
ADP-ribosylation factor-like protein 8
UniProt Gene Name
ARL5B
UniProt Synonym Gene Names
ARL8
UniProt Entry Name
ARL5B_HUMAN
NCBI Protein Information
ADP-ribosylation factor-like protein 5B; ADP-ribosylation factor-like 8; ADP-ribosylation factor-like protein 8; ADP-ribosylation-like factor 8

UniProt Comments for ARL8

ARL5B: Binds and exchanges GTP and GDP. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 10p12.31

Cellular Component: intracellular

Molecular Function: GTP binding

Biological Process: small GTPase mediated signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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