Goat Anti-ACAP1 Polyclonal Antibody – Cat# MBS627902

Western blot analysis of ACAP1 in Jurkat lysate (RIPA buffer, 35μg total protein per lane) using ACAP1 antibody (0.5μg/ml).

Centaurin, Beta 1 (ACAP1) Antibody – Goat Polyclonal

Name: ACAP1 Antibody; Polyclonal; Goat; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS627902
Price: 980 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627902

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6… More details

$980 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Centaurin, beta 1 (ACAP1), Polyclonal Antibody

Full Product Name

Centaurin, beta 1 (CENTB1, ACAP1, ACAP1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1)

Product Gene Name

anti-ACAP1 antibody

Product Synonym Names

Anti -Centaurin, beta 1 (CENTB1, ACAP1, ACAP1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1)

Clonality

Polyclonal

Host

Goat

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human ACAP1 at ~80-90kD in Jurkat lysates (predicted MW of 85kDa according to NP_055531). Species Crossreactivity: Mouse and rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

81,536 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (7239848..7254793). Location: 17p13.1

OMIM

607763

3D Structure

ModBase 3D Structure for Q15027

Immunogen

Synthetic peptide of ACAP1.

Product Categories/Family for anti-ACAP1 antibody

Antibodies; Abs to Nuclear Proteins

Applications Tested/Suitable for anti-ACAP1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-ACAP1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32000
Western Blot: 0.5-2ug/ml

Other Notes

Small volumes of anti-ACAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACAP1 purchase

MBS627902 is an antibody targeting ACAP1. Also known as Centaurin, beta 1 (CENTB1, ACAP1, ACAP1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1). Reactive in Human, Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ACAP1. It may not necessarily be applicable to this product.

NCBI GI #

32879919

NCBI GeneID

9744

UniProt Primary Accession #

Q15027

UniProt Secondary Accession #

Q53XN9

UniProt Related Accession #

Q15027

NCBI Official Full Name

centaurin, beta 1

NCBI Official Synonym Full Names

ArfGAP with coiled-coil, ankyrin repeat and PH domains 1

NCBI Official Symbol

ACAP1

NCBI Official Synonym Symbols

CENTB1; KIAA0050

UniProt Protein Name

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

UniProt Synonym Protein Names

Centaurin-beta-1

Protein Family

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein

UniProt Gene Name

ACAP1

UniProt Synonym Gene Names

CENTB1; KIAA0050

UniProt Entry Name

ACAP1_HUMAN

NCBI Protein Information

arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; cnt-b1; centaurin-beta-1; centaurin, beta 1; OTTHUMP00000128386; Arf GAP with coiled coil, ANK repeat and PH domains 1

UniProt Comments for ACAP1

CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; recycling endosome membrane

Molecular Function: zinc ion binding

Biological Process: protein transport; positive regulation of GTPase activity

Research Articles on ACAP1

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACAP1 antibody

Products by Pathway	Pathway Diagram
Arf6 Signaling Events Pathway antibodies	Arf6 Signaling Events Pathway Diagram
Arf6 Trafficking Events Pathway antibodies	Arf6 Trafficking Events Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram

Diseases associated with anti-ACAP1 antibody

Disease Name	Pubmed Publications
Central Nervous System Neoplasms Antibodies	>1 publications with ACAP1 and Central Nervous System Neoplasms
Nervous System Neoplasms Antibodies	>1 publications with ACAP1 and Nervous System Neoplasms
Brain Diseases Antibodies	>1 publications with ACAP1 and Brain Diseases
Necrosis Antibodies	>1 publications with ACAP1 and Necrosis

Organs/Tissues associated with anti-ACAP1 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>1 publications with ACAP1 and Nerve
Muscle Antibodies	>1 publications with ACAP1 and Muscle
Heart Antibodies	>1 publications with ACAP1 and Heart
Lung Antibodies	>1 publications with ACAP1 and Lung
Brain Antibodies	>1 publications with ACAP1 and Brain
Blood Antibodies	>1 publications with ACAP1 and Blood