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Raf Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628411

Raf-1, a mitogen-activated protein kinase, is the main effector recruited by GTP-bound Ras in order to activate the MAP kinase pathway (1). 14-3-3 binds to c-Raf at two phospho-serine residues: Ser259 and Ser621 (2). Mutation of Ser259 to alanine… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Raf, Polyclonal Antibody
Full Product Name
Raf, phosphorylated (Ser259) (Raf-1, c-Raf)
Product Synonym Names
Anti -Raf, phosphorylated (Ser259) (Raf-1, c-Raf)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Chicken, Human, Mouse, Rat, Xenopus
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes endogenous levels of human c-Raf only when phosphorylated at Ser259. Species crossreactivity: mouse, rat, chicken and Xenopus.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser259 of human c-Raf.
Applications Tested/Suitable for anti-Raf antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Raf antibody
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50

Western Blot (WB) of anti-Raf antibody

Site specificity of Phospho-Raf (Ser259) Antibody: Western blot analysis of recombinant Myc-tagged c-Raf protein, wild-type (lanes 1 and 3) and S259A mutant (lanes 2 and 4), using Phospho-Raf (Ser259) Antibody or a Myc antibody. (Provided by Dr. Guri Tzivion, Massachusetts General Hospital.)
Rabbit Anti-Raf Polyclonal Antibody – Cat# MBS628411

Western Blot (WB) of anti-Raf antibody

Western blot analysis of extracts from HeLa cells, untreated or TPA-treated, using Phospho-Raf (Ser259) Antibody (upper), or a total c-Raf antibody (lower).
Rabbit Anti-Raf Polyclonal Antibody, Western Blot (WB) – Cat# MBS628411

Immunohistochemistry (IHC) of anti-Raf antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing cytoplasmic and membrane localization of phosphorylated Raf, using Phospho-Raf (Ser259) Antibody.
Rabbit Anti-Raf Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628411

Other Notes

Small volumes of anti-Raf antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Raf purchase

MBS628411 is an antibody targeting Raf. Also known as Raf, phosphorylated (Ser259) (Raf-1, c-Raf). Reactive in Chicken, Human, Mouse, Rat, Xenopus. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Raf. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
RAF

UniProt Comments for Raf

Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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