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ALOX12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628650

MBS628650 is an antibody targeting ALOX12. Also known as ALOX12, CT (Platelet-type Lipoxygenase 12, Arachidonate 12-lipoxygenase, 12S-type, 12S-lipoxygenase, 12S-LOX, LOG12). Reactive in Human. Host: Rabbit. Oxygenase and 14,15-leukotriene A4 synthase… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALOX12, Polyclonal Antibody
Full Product Name
ALOX12, CT (Platelet-type Lipoxygenase 12, Arachidonate 12-lipoxygenase, 12S-type, 12S-lipoxygenase, 12S-LOX, LOG12)
Product Gene Name
anti-ALOX12 antibody
Product Synonym Names
Anti -ALOX12, CT (Platelet-type Lipoxygenase 12, Arachidonate 12-lipoxygenase, 12S-type, 12S-lipoxygenase, 12S-LOX, LOG12)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ALOX12.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
75,694 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (6899384..6914055). Location: 17p13.1
OMIM
152391
3D Structure
ModBase 3D Structure for P18054
Immunogen
Synthetic peptide selected from the C-terminal region of human ALOX12 (KLH).

Product Categories/Family for anti-ALOX12 antibody

Antibodies; Abs to Leukotrienes
Applications Tested/Suitable for anti-ALOX12 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ALOX12 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-ALOX12 antibody

Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
Rabbit Anti-ALOX12 Polyclonal Antibody – Cat# MBS628650

Immunohistochemistry (IHC) of anti-ALOX12 antibody

Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ALOX12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628650

Immunofluorescence (IF) of anti-ALOX12 antibody

Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-ALOX12 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS628650

Flow Cytometry (FC/FACS) of anti-ALOX12 antibody

ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ALOX12 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS628650

Other Notes

Small volumes of anti-ALOX12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALOX12. It may not necessarily be applicable to this product.

NCBI GI #
154426292
NCBI GeneID
239
NCBI Accession #
NP_000688.2
UniProt Primary Accession #
P18054
UniProt Secondary Accession #
O95569; Q6ISF8; Q9UQM4
UniProt Related Accession #
P18054; Q16120
GenBank Nucleotide #
NM_000697.2
NCBI Official Full Name
arachidonate 12-lipoxygenase, 12S-type
NCBI Official Synonym Full Names
arachidonate 12-lipoxygenase
NCBI Official Symbol
ALOX12
NCBI Official Synonym Symbols
LOG12; 12-LOX; 12S-LOX
UniProt Protein Name
Arachidonate 12-lipoxygenase, 12S-type
UniProt Synonym Protein Names
Platelet-type lipoxygenase 12
Protein Family
Arachidonate 12-lipoxygenase
UniProt Gene Name
ALOX12
UniProt Synonym Gene Names
LOG12
UniProt Entry Name
LOX12_HUMAN
NCBI Protein Information
arachidonate 12-lipoxygenase, 12S-type; platelet 12-LOX; 12S-lipoxygenase; OTTHUMP00000128281; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12

UniProt Comments for ALOX12

ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.

Protein type: Apoptosis; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.31

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; sarcolemma

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; hepoxilin A3 synthase activity; protein binding; arachidonate 12-lipoxygenase activity; lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; linoleic acid metabolic process; positive regulation of caspase activity; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; lipoxygenase pathway; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; hepoxilin metabolic process; superoxide release; aging; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on ALOX12

1. the rs2073438 polymorphism of ALOX12 contributes to the variation of obesity phenotypes in young Chinese men

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALOX12 antibody

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-ALOX12 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with ALOX12 and Neoplasms
 Cardiovascular Diseases Antibodies  >5 publications with ALOX12 and Cardiovascular Diseases
 Skin Diseases Antibodies  >5 publications with ALOX12 and Skin Diseases
 Congenital Abnormalities Antibodies  >4 publications with ALOX12 and Congenital Abnormalities
 Atherosclerosis Antibodies  >4 publications with ALOX12 and Atherosclerosis
 Osteoporosis Antibodies  >4 publications with ALOX12 and Osteoporosis
 Leukemia Antibodies  >2 publications with ALOX12 and Leukemia
 Drug Toxicity Antibodies  >2 publications with ALOX12 and Drug Toxicity
 Diabetes Mellitus, Type 2 Antibodies  >2 publications with ALOX12 and Diabetes Mellitus, Type 2
 Coronary Disease Antibodies  >2 publications with ALOX12 and Coronary Disease

Organs/Tissues associated with anti-ALOX12 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >5 publications with ALOX12 and Skin
 Ovary Antibodies  >1 publications with ALOX12 and Ovary
 Lung Antibodies  >1 publications with ALOX12 and Lung
 Embryonic Tissue Antibodies  >1 publications with ALOX12 and Embryonic Tissue
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