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PRDM14 Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629000

PRDM14 (PR [PRDI-BF1 and RIZ] domain containing protein 14) is a 64kD (predicted) member of the PR Domain family of proteins. It is expressed in embryonic stem cells and multiple tumor cell types. PRDM14 likely demonstrates histone methyltransferase activity,… More details

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Product Overview

Online Datasheet in PDF
Product Name
PRDM14, Polyclonal Antibody
Full Product Name
PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14)
Product Gene Name
anti-PRDM14 antibody
Product Synonym Names
Anti -PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14)
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PRDM14.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
64,062 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (70964021..70983562, complement). Location: 8q13.3
3D Structure
ModBase 3D Structure for Q9GZV8
Immunogen
Purified, E. coli-derived, recombinant human PRDM14 (aa2-201; Accession #Q9GZV8).

Product Categories/Family for anti-PRDM14 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-PRDM14 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PRDM14 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 0.1ug/ml

SDS-Page of anti-PRDM14 antibody

Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.1ug/ml sheep anti-hPRDM14.
Sheep Anti-PRDM14 Polyclonal Antibody – Cat# MBS629000

Other Notes

Small volumes of anti-PRDM14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRDM14 purchase

MBS629000 is an antibody targeting PRDM14. Also known as PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14). Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for PRDM14. It may not necessarily be applicable to this product.

NCBI GI #
13375636
NCBI GeneID
63978
NCBI Accession #
NP_078780.1
UniProt Primary Accession #
Q9GZV8
UniProt Secondary Accession #
Q86UX9
UniProt Related Accession #
Q9GZV8
GenBank Nucleotide #
NM_024504.2
NCBI Official Full Name
PR domain zinc finger protein 14
NCBI Official Synonym Full Names
PR domain containing 14
NCBI Official Symbol
PRDM14
NCBI Official Synonym Symbols
PFM11; MGC59730
UniProt Protein Name
PR domain zinc finger protein 14
UniProt Synonym Protein Names
PR domain-containing protein 14
Protein Family
PR domain zinc finger protein
UniProt Gene Name
PRDM14
UniProt Entry Name
PRD14_HUMAN
NCBI Protein Information
PR domain zinc finger protein 14; OTTHUMP00000197599; PR domain-containing protein 14; PR-domain zinc finger protein 14

NCBI Summary for PRDM14

The PR domain is a protein-protein interaction module of about 100 amino acids. PR domain-containing proteins, such as PRDM14, are often involved in transcriptional regulation (Jiang and Huang, 2000 [PubMed 10668202]).[supplied by OMIM]

UniProt Comments for PRDM14

PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Protein type: Methyltransferase, protein arginine, predicted; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: nucleoplasm

Molecular Function: methyltransferase activity; protein binding; DNA binding; metal ion binding

Biological Process: fertilization; transcription, DNA-dependent; regulation of transcription, DNA-dependent; homeostasis of number of cells within a tissue; somatic stem cell maintenance; negative regulation of fibroblast growth factor receptor signaling pathway; inner cell mass cell fate commitment; cell morphogenesis; germ-line stem cell maintenance; cell fate specification; regulation of gene expression, epigenetic; germ cell development
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Research Articles on PRDM14

1. The high expression of PRDM14 in non-small cell lung cancer is associated with differentiation and histological type.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PRDM14 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4 publications with PRDM14 and Breast Neoplasms
 Carcinoma in Situ Antibodies  >2 publications with PRDM14 and Carcinoma in Situ

Organs/Tissues associated with anti-PRDM14 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with PRDM14 and Testis
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