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NME2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629907

NM2 is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase.… More details

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Product Overview

Online Datasheet in PDF
Product Name
NME2, Polyclonal Antibody
Full Product Name
NME2, NT (Nucleoside Diphosphate Kinase B, NDK B, NDP Kinase B, nm23-H2, C-myc Purine-binding Transcription Factor PUF, NM23B)
Product Gene Name
anti-NME2 antibody
Product Synonym Names
Anti -NME2, NT (Nucleoside Diphosphate Kinase B, NDK B, NDP Kinase B, nm23-H2, C-myc Purine-binding Transcription Factor PUF, NM23B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NME2. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
17,298 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (49242891..49249105). Location: 17q21.3
3D Structure
ModBase 3D Structure for Q6FHN3
Immunogen
Synthetic peptide selected from the N-terminal region of human NME2 (KLH).

Product Categories/Family for anti-NME2 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-NME2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NME2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-NME2 antibody

NME2 Antibody (F40) western blot analysis in HL-60,Jurkat,MCF-7 cell line,mouse kidney and rat stomach tissue lysates (35ug/lane).This demonstrates the NME2 antibody detected the NME2 protein (arrow).
Rabbit Anti-NME2 Polyclonal Antibody – Cat# MBS629907

Immunohistochemistry (IHC) of anti-NME2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-NME2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629907

Immunofluorescence (IF) of anti-NME2 antibody

Fluorescent confocal image of Hela cell stained with NME2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NME2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NME2 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Rabbit Anti-NME2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS629907

Other Notes

Small volumes of anti-NME2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NME2 purchase

MBS629907 is an antibody targeting NME2. Also known as NME2, NT (Nucleoside Diphosphate Kinase B, NDK B, NDP Kinase B, nm23-H2, C-myc Purine-binding Transcription Factor PUF, NM23B). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NME2. It may not necessarily be applicable to this product.

NCBI GI #
49456397
NCBI GeneID
4831
UniProt Primary Accession #
Q6FHN3
UniProt Related Accession #
P22392; Q6FHN3
NCBI Official Full Name
NME2
NCBI Official Synonym Full Names
non-metastatic cells 2, protein (NM23B) expressed in
NCBI Official Symbol
NME2
NCBI Official Synonym Symbols
PUF; NDKB; NDPKB; NM23B; NDPK-B; MGC2212; NM23-H2; MGC111212
UniProt Protein Name
Nucleoside diphosphate kinase
UniProt Gene Name
NME2
UniProt Entry Name
Q6FHN3_HUMAN
NCBI Protein Information
nucleoside diphosphate kinase B; NDP kinase B; OTTHUMP00000174727; OTTHUMP00000174728; OTTHUMP00000174774; OTTHUMP00000174775; OTTHUMP00000174776; c-myc transcription factor; histidine protein kinase NDKB; c-myc purine-binding transcription factor PUF; non-metastatic cells 2, protein (NM23) expressed in

NCBI Summary for NME2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]
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UniProt Comments for NME2

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; EC 2.7.13.3; Kinase, nucleoside diphosphate; Kinase, other; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; membrane; mitochondrion; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; gamma-tubulin binding; protein histidine kinase activity; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; protein kinase binding; transcription factor activity; single-stranded DNA binding; ATP binding

Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; response to amine stimulus; negative regulation of apoptosis
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Research Articles on NME2

1. Studies indicate that most of the proteins known to interact with human NME proteins were also found in starlet sea anemone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NME2 antibody

 Reference Product  PubMed Publications
 NME3 antibody  >4 publications with NME2 and NME3

Pathways associated with anti-NME2 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nucleotide Metabolism Pathway antibodies  Nucleotide Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway antibodies  Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies  Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram
 Pyrimidine Ribonucleotides Interconversion Pathway antibodies  Pyrimidine Ribonucleotides Interconversion Pathway Diagram

Diseases associated with anti-NME2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >24 publications with NME2 and Neoplasms
 Carcinoma Antibodies  >11 publications with NME2 and Carcinoma
 Neoplasm Metastasis Antibodies  >7 publications with NME2 and Neoplasm Metastasis
 Drug Toxicity Antibodies  >6 publications with NME2 and Drug Toxicity
 Female Urogenital Diseases Antibodies  >4 publications with NME2 and Female Urogenital Diseases
 Breast Neoplasms Antibodies  >4 publications with NME2 and Breast Neoplasms
 Genital Neoplasms, Female Antibodies  >4 publications with NME2 and Genital Neoplasms, Female
 Carcinoma, Squamous Cell Antibodies  >4 publications with NME2 and Carcinoma, Squamous Cell
 Anemia Antibodies  >3 publications with NME2 and Anemia
 Melanoma Antibodies  >3 publications with NME2 and Melanoma

Organs/Tissues associated with anti-NME2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >6 publications with NME2 and Liver
 Lung Antibodies  >6 publications with NME2 and Lung
 Heart Antibodies  >6 publications with NME2 and Heart
 Blood Antibodies  >5 publications with NME2 and Blood
 Bone Marrow Antibodies  >4 publications with NME2 and Bone Marrow
 Muscle Antibodies  >4 publications with NME2 and Muscle
 Bone Antibodies  >4 publications with NME2 and Bone
 Embryonic Tissue Antibodies  >3 publications with NME2 and Embryonic Tissue
 Brain Antibodies  >3 publications with NME2 and Brain
 Pancreas Antibodies  >2 publications with NME2 and Pancreas
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