• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630385

MBS630385 is an antibody targeting H3. Also known as Histone H3, phosphorylated (Ser10) (Mitosis Marker). Reactive in Drosophila, Human, Th, Xenopus. Host: Rabbit. More details

$1265 / 0.2 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Histone H3, phosphorylated (Ser10) (Mitosis Marker)
Product Gene Name
Product Synonym Names
Anti -Histone H3, phosphorylated (Ser10) (Mitosis Marker)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Drosophila, Human, Th, Xenopus
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Linear peptide coresponding to human Histone H3 at Ser10

Product Categories/Family for anti-H3 antibody

Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-H3 antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells.

Testing Data of anti-H3 antibody

Lane 1: Normal HeLa nuclear extract Lane 2: Acid-extracted protein from colcemid-arrested HeLa cells Resolved by electorphoresis, transferred to nitrocellulose and probed with MBS630385 (0.5mg/ml).
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS630385

Testing Data of anti-H3 antibody

Rabbit Anti-H3 Polyclonal Antibody, Testing Data – Cat# MBS630385

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
Protein Family
NCBI Protein Information
histone H3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000