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EIF4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630477

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA… More details

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Product Overview

Online Datasheet in PDF
Product Name
EIF4B, Polyclonal Antibody
Full Product Name
EIF4B, ID (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG_2014609, eIF4B)
Product Gene Name
anti-EIF4B antibody
Product Synonym Names
Anti -EIF4B, ID (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG_2014609, eIF4B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human EIF4B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
69,151 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (53400062..53435993). Location: 12q13.13
OMIM
603928
3D Structure
ModBase 3D Structure for P23588
Immunogen
Synthetic peptide selected from the center region of human EIF4B (KLH).

Product Categories/Family for anti-EIF4B antibody

Antibodies; Abs to Translation Initiation Factors
Applications Tested/Suitable for anti-EIF4B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF4B antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-EIF4B antibody

Western blot analysis of anti-EIF4B Antibody (Center) Pab in HepG2 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab.
Rabbit Anti-EIF4B Polyclonal Antibody – Cat# MBS630477

Western Blot (WB) of anti-EIF4B antibody

Western blot analysis of anti-EIF4B Antibody (Center) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab.
Rabbit Anti-EIF4B Polyclonal Antibody, Western Blot (WB) – Cat# MBS630477

Immunohistochemistry (IHC) of anti-EIF4B antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-EIF4B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630477

Other Notes

Small volumes of anti-EIF4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4B purchase

MBS630477 is an antibody targeting EIF4B. Also known as EIF4B, ID (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG_2014609, eIF4B). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4B. It may not necessarily be applicable to this product.

NCBI GI #
50053795
NCBI GeneID
1975
NCBI Accession #
NP_001408.2
UniProt Primary Accession #
P23588
UniProt Secondary Accession #
Q4G0E3; Q53HQ2; Q6GPH5; Q6IB46; Q8WYK5
UniProt Related Accession #
P23588; Q6AI40; Q7Z5Y0; Q9P192
GenBank Nucleotide #
NM_001417.4
NCBI Official Full Name
eukaryotic translation initiation factor 4B
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4B
NCBI Official Symbol
EIF4B
NCBI Official Synonym Symbols
EIF-4B; PRO1843
UniProt Protein Name
Eukaryotic translation initiation factor 4B
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF4B
UniProt Entry Name
IF4B_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 4B

UniProt Comments for EIF4B

EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Protein type: Translation initiation; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; RNA binding; translation initiation factor activity; nucleotide binding

Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; regulation of translational initiation
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Research Articles on EIF4B

1. Studies indicate that eIF4A (DDX2), together with its accessory proteins eIF4B and eIF4H, is thought to act as a helicase that unwinds secondary structures in the mRNA 5' UTR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4B antibody

 Reference Product  PubMed Publications
 EIF4E antibody  >39 publications with EIF4B and EIF4E
 CASP3 antibody  >3 publications with EIF4B and CASP3
 SFN antibody  >1 publications with EIF4B and SFN
 EIF3A antibody  >1 publications with EIF4B and EIF3A

Pathways associated with anti-EIF4B antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram

Diseases associated with anti-EIF4B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >19 publications with EIF4B and Neoplasms
 Necrosis Antibodies  >8 publications with EIF4B and Necrosis
 Cell Transformation, Neoplastic Antibodies  >6 publications with EIF4B and Cell Transformation, Neoplastic
 Hepatitis Antibodies  >4 publications with EIF4B and Hepatitis
 Breast Neoplasms Antibodies  >3 publications with EIF4B and Breast Neoplasms
 Carcinoma Antibodies  >3 publications with EIF4B and Carcinoma
 Melanoma Antibodies  >2 publications with EIF4B and Melanoma
 Kidney Diseases Antibodies  >2 publications with EIF4B and Kidney Diseases
 Adenocarcinoma Antibodies  >2 publications with EIF4B and Adenocarcinoma
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >1 publications with EIF4B and Precursor Cell Lymphoblastic Leukemia-Lymphoma

Organs/Tissues associated with anti-EIF4B antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >36 publications with EIF4B and Blood
 Mouth Antibodies  >10 publications with EIF4B and Mouth
 Liver Antibodies  >7 publications with EIF4B and Liver
 Vascular Antibodies  >4 publications with EIF4B and Vascular
 Muscle Antibodies  >4 publications with EIF4B and Muscle
 Eye Antibodies  >2 publications with EIF4B and Eye
 Prostate Antibodies  >2 publications with EIF4B and Prostate
 Brain Antibodies  >2 publications with EIF4B and Brain
 Lung Antibodies  >2 publications with EIF4B and Lung
 Kidney Antibodies  >2 publications with EIF4B and Kidney
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