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SMAD4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631176

SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate… More details

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Product Overview

Online Datasheet in PDF
Product Name
SMAD4, Polyclonal Antibody
Full Product Name
SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4)
Product Gene Name
anti-SMAD4 antibody
Product Synonym Names
Anti -SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human SMAD4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,085 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (48556583..48611412). Location: 18q21.1
3D Structure
ModBase 3D Structure for Q9BYG6
Immunogen
Synthetic peptide selected from the C-terminal region of human SMAD4 (KLH).

Product Categories/Family for anti-SMAD4 antibody

Antibodies; Abs to Smad Proteins
Applications Tested/Suitable for anti-SMAD4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-SMAD4 antibody
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Flow cytometry: 1:10~1:50
Immunofluorescence: 1:10~1:50

Western Blot (WB) of anti-SMAD4 antibody

Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) .
Rabbit Anti-SMAD4 Polyclonal Antibody – Cat# MBS631176

Immunohistochemistry (IHC) of anti-SMAD4 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-SMAD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631176

Immunofluorescence (IF) of anti-SMAD4 antibody

Fluorescent confocal image of Hela cell stained with SMAD4 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Rabbit Anti-SMAD4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS631176

Flow Cytometry (FC/FACS) of anti-SMAD4 antibody

SMAD4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SMAD4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS631176

Other Notes

Small volumes of anti-SMAD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMAD4 purchase

MBS631176 is an antibody targeting SMAD4. Also known as SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMAD4. It may not necessarily be applicable to this product.

NCBI GI #
13603414
NCBI GeneID
4089
UniProt Primary Accession #
Q9BYG6
UniProt Related Accession #
Q13485; Q9BYG6
NCBI Official Full Name
SMAD4
NCBI Official Synonym Full Names
SMAD family member 4
NCBI Official Symbol
SMAD4
NCBI Official Synonym Symbols
JIP; DPC4; MADH4
UniProt Protein Name
SMAD4
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD4
UniProt Entry Name
Q9BYG6_HUMAN
NCBI Protein Information
mothers against decapentaplegic homolog 4; MAD homolog 4; OTTHUMP00000163548; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4

NCBI Summary for SMAD4

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]
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UniProt Comments for SMAD4

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; centrosome; transcription factor complex; cytoplasm; nuclear chromatin; cytosol; nucleus

Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: developmental growth; axon guidance; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; palate development; negative regulation of transcription from RNA polymerase II promoter; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; transcription, DNA-dependent; regulation of binding; in utero embryonic development; neuron fate commitment; positive regulation of transforming growth factor beta receptor signaling pathway; gastrulation with mouth forming second; somite rostral/caudal axis specification; formation of anatomical boundary; SMAD protein complex assembly; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; regulation of hair follicle development; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; endoderm development

Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome
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Research Articles on SMAD4

1. Smad4 expression was related with tumor differentiation and Lauren classification of gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMAD4 antibody

 Reference Product  PubMed Publications
 SMAD2 antibody  >762 publications with SMAD4 and SMAD2
 SMAD3 antibody  >694 publications with SMAD4 and SMAD3
 SMAD1 antibody  >232 publications with SMAD4 and SMAD1
 SMAD5 antibody  >94 publications with SMAD4 and SMAD5
 CTNNB1 antibody  >85 publications with SMAD4 and CTNNB1
 JUN antibody  >69 publications with SMAD4 and JUN
 CDKN1A antibody  >61 publications with SMAD4 and CDKN1A
 SKI antibody  >47 publications with SMAD4 and SKI
 SP1 antibody  >36 publications with SMAD4 and SP1
 FOXH1 antibody  >29 publications with SMAD4 and FOXH1

Pathways associated with anti-SMAD4 antibody

 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 ALK2 Signaling Events Pathway antibodies  ALK2 Signaling Events Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram

Diseases associated with anti-SMAD4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >916 publications with SMAD4 and Neoplasms
 Carcinoma Antibodies  >509 publications with SMAD4 and Carcinoma
 Gastrointestinal Neoplasms Antibodies  >316 publications with SMAD4 and Gastrointestinal Neoplasms
 Adenocarcinoma Antibodies  >300 publications with SMAD4 and Adenocarcinoma
 Pancreatic Neoplasms Antibodies  >294 publications with SMAD4 and Pancreatic Neoplasms
 Intestinal Diseases Antibodies  >257 publications with SMAD4 and Intestinal Diseases
 Colorectal Neoplasms Antibodies  >244 publications with SMAD4 and Colorectal Neoplasms
 Fibrosis Antibodies  >117 publications with SMAD4 and Fibrosis
 Adenoma Antibodies  >100 publications with SMAD4 and Adenoma
 Liver Diseases Antibodies  >89 publications with SMAD4 and Liver Diseases

Organs/Tissues associated with anti-SMAD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >356 publications with SMAD4 and Bone
 Liver Antibodies  >150 publications with SMAD4 and Liver
 Pancreas Antibodies  >127 publications with SMAD4 and Pancreas
 Blood Antibodies  >119 publications with SMAD4 and Blood
 Lung Antibodies  >111 publications with SMAD4 and Lung
 Muscle Antibodies  >110 publications with SMAD4 and Muscle
 Embryonic Tissue Antibodies  >104 publications with SMAD4 and Embryonic Tissue
 Vascular Antibodies  >94 publications with SMAD4 and Vascular
 Kidney Antibodies  >81 publications with SMAD4 and Kidney
 Skin Antibodies  >72 publications with SMAD4 and Skin
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