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CD3, Epsilon (cd3e) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632085

CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse… More details

$420 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3, epsilon (cd3e), Monoclonal Antibody
Full Product Name
CD3, epsilon (Early T Cell Marker) (FITC)
Product Gene Name
Product Synonym Names
Anti -CD3, epsilon (Early T Cell Marker) (FITC)
Clonality
Monoclonal
Isotype
IgG2c,k
Clone Number
5K4
Host
Rat
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.4, sodium azide. Conjugated to FITC.
Purity/Purification
Purified
Purified
Specificity
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-cd3e antibody

Applications Tested/Suitable for anti-cd3e antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-cd3e antibody
Identification and enumeration of CD3+ cells by flow cytometry 1, 2, , Immunoprecipitation 1, , In vitro depletion of CD3+ cells 1, , In vitro activation of T cells (accessory cells must be provided) 1, 3, 4, , Immunohistochemistry 5
Dilution: Flow Cytometry: 3ug/10e6 cells

Flow Cytometry (FC/FACS) of anti-cd3e antibody

Cells from BALB/c mesenteric lymph nodes were double-stained with C2400-26R3 and MBS632085. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer
Rat Anti-cd3e Monoclonal Antibody – Cat# MBS632085

Other Notes

Small volumes of anti-cd3e antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for cd3e purchase

MBS632085 is an antibody targeting cd3e. Also known as CD3, epsilon (Early T Cell Marker) (FITC). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for cd3e. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD3 epsilon
NCBI Official Symbol
cd3e
NCBI Protein Information
CD3 epsilon

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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