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PLEKHA4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6334809

PLEKHA4 binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLEKHA4, Polyclonal Antibody
Full Product Name
PLEKHA4, NT (PLEKHA4, PEPP1, Pleckstrin homology domain-containing family A member 4, Phosphoinositol 3-phosphate-binding protein 1) (FITC)
Product Gene Name
anti-PLEKHA4 antibody
Product Synonym Names
PLEKHA4; PEPP1; Pleckstrin homology domain-containing family A member 4; Phosphoinositol 3-phosphate-binding protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
AY007233 mRNA
3D Structure
ModBase 3D Structure for Q9H4M7
Immunogen
PLEKHA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-58 amino acids from the N-terminal region of human PLEKHA4.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-PLEKHA4 antibody

Antibodies; Pleckstrin Homology Proteins
Applications Tested/Suitable for anti-PLEKHA4 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PLEKHA4 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PLEKHA4 antibody

PLEKHA4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PLEKHA4 antibody detected the PLEKHA4 protein (arrow).
Rabbit Anti-PLEKHA4 Polyclonal Antibody – Cat# MBS6334809

Flow Cytometry (FC/FACS) of anti-PLEKHA4 antibody

PLEKHA4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PLEKHA4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6334809

Immunohistochemistry (IHC) of anti-PLEKHA4 antibody

PLEKHA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PLEKHA4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6334809

Product References and Citations for anti-PLEKHA4 antibody

Stelzl, U., et al. Cell 122(6):957-968(2005). Dowler, S., et al. Biochem. J. 351 (PT 1), 19-31 (2000).

Other Notes

Small volumes of anti-PLEKHA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLEKHA4 purchase

MBS6334809 is an antibody targeting PLEKHA4. Also known as PLEKHA4, NT (PLEKHA4, PEPP1, Pleckstrin homology domain-containing family A member 4, Phosphoinositol 3-phosphate-binding protein 1) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PLEKHA4. It may not necessarily be applicable to this product.

NCBI GI #
238859651
NCBI GeneID
57664
NCBI Accession #
NP_065955.2
NCBI Related Accession #
NP_001154826.1
UniProt Primary Accession #
Q9H4M7
UniProt Related Accession #
Q9H4M7
GenBank Nucleotide #
NP_065955.2
NCBI Official Full Name
pleckstrin homology domain-containing family A member 4 isoform 1
NCBI Official Synonym Full Names
pleckstrin homology domain containing A4
NCBI Official Symbol
PLEKHA4
NCBI Official Synonym Symbols
PEPP1
UniProt Protein Name
Pleckstrin homology domain-containing family A member 4
UniProt Synonym Protein Names
Phosphoinositol 3-phosphate-binding protein 1; PEPP-1
Protein Family
Pleckstrin homology domain-containing family
UniProt Gene Name
PLEKHA4
UniProt Synonym Gene Names
PEPP1; PH domain-containing family A member 4; PEPP-1
NCBI Protein Information
pleckstrin homology domain-containing family A member 4

NCBI Summary for PLEKHA4

This gene encodes a pleckstrin homology (PH) domain-containing protein. The PH domain is found near the N-terminus and contains a putative phosphatidylinositol 3, 4, 5-triphosphate-binding motif (PPBM). Elevated expression of this gene has been observed in some melanomas. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2017]
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UniProt Comments for PLEKHA4

Binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P), but not to other phosphoinositides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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