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PMCH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335148

MBS6335148 is an antibody targeting PMCH. Also known as PMCH, ID (PMCH, MCH, Pro-MCH, Neuropeptide-glycine-glutamic acid, Neuropeptide-glutamic acid-isoleucine, Melanin-concentrating hormone) (APC). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PMCH, Polyclonal Antibody
Full Product Name
PMCH, ID (PMCH, MCH, Pro-MCH, Neuropeptide-glycine-glutamic acid, Neuropeptide-glutamic acid-isoleucine, Melanin-concentrating hormone) (APC)
Product Gene Name
Product Synonym Names
PMCH; MCH; Pro-MCH; Neuropeptide-glycine-glutamic acid; Neuropeptide-glutamic acid-isoleucine; Melanin-concentrating hormone
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human PMCH. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
18,679 Da
OMIM
176795
3D Structure
ModBase 3D Structure for P20382
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 94-122 in the cenrtral terminal region of human PMCH.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-PMCH antibody

Applications Tested/Suitable for anti-PMCH antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-PMCH antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PMCH antibody

Western Blot analysis in K562 cell line lysates (15ug/lane) usingMBS6008589.This demonstrates that MBS6008589 detected the PMCH protein (arrow).
Rabbit Anti-PMCH Polyclonal Antibody – Cat# MBS6335148

Western Blot (WB) of anti-PMCH antibody

Western Blot analysis in mouse brain tissue lysates (15ug/lane) usingMBS6008589.This demonstrates that MBS6008589 detected the PMCH protein (arrow).
Rabbit Anti-PMCH Polyclonal Antibody, Western Blot (WB) – Cat# MBS6335148

Western Blot (WB) of anti-PMCH antibody

Western Blot analysis of PMCH in 293 cell lysates (2ug/lane) usingMBS6008589. Lane 1: nontransfected Lane 2: transiently transfected with the PMCH gene.
Rabbit Anti-PMCH Polyclonal Antibody, Western Blot (WB) – Cat# MBS6335148

Immunohistochemistry (IHC) of anti-PMCH antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using MBS6008589 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008589 for immunohistochemistry.
Rabbit Anti-PMCH Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6335148

Flow Cytometry (FC/FACS) of anti-PMCH antibody

Flow Cytometric analysis of K562 cells using MBS6008589 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PMCH Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6335148

Product References and Citations for anti-PMCH antibody

1. Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010). 2. Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009). 3. Aziz, A., et al. Brain Pathol. 18(4):474-483(2008). 4. Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008).

Other Notes

Small volumes of anti-PMCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PMCH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pro-MCH preproprotein
NCBI Official Synonym Full Names
pro-melanin concentrating hormone
NCBI Official Symbol
PMCH
NCBI Official Synonym Symbols
MCH; ppMCH
UniProt Protein Name
Pro-MCH
Protein Family
UniProt Gene Name
PMCH
UniProt Synonym Gene Names
MCH; NGE; Neuropeptide G-E; NEI; Neuropeptide E-I; MCH
UniProt Entry Name
MCH_HUMAN
NCBI Protein Information
pro-MCH

NCBI Summary for PMCH

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include melanin-concentrating hormone (MCH), neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE). Melanin-concentrating hormone is a 19-amino acid neuropeptide that stimulates hunger and may additionally regulate energy homeostasis, reproductive function, and sleep. Pseudogenes of this gene have been identified on chromosome 5. [provided by RefSeq, Jul 2015]
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UniProt Comments for PMCH

PMCH: MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation. Belongs to the melanin-concentrating hormone family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q23-q24
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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