Mouse Anti-BTLA Monoclonal Antibody – Cat# MBS633646

Flow Cytometry analysis of human PBL using MBS633646

BTLA Antibody – Mouse Monoclonal

Name: BTLA Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS633646
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633646

Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270… More details

$830 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BTLA, Monoclonal Antibody

Full Product Name

BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC)

Product Synonym Names

Anti -BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC)

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

11K3

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).

Purity/Purification

Purified by Protein A affinity chromatography form tissue culture supernatant.

Concentration

0.25 mg/ml (lot specific)

Specificity

Recognizes human CD272 (BTLA). Binds to CD272 on the cell surface of human PBMC in Flow Cytometry and to recombinant CD272-muIg in ELISA. Does not block binding of Biotinylated CD270 (HVEM)-muIg to CD272-muIg in ELISA, but appears to enhance this interaction.

Preparation and Storage

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to soluble human BTLA

Product Categories/Family for anti-BTLA antibody

Antibodies; Abs to CD Markers

Applications Tested/Suitable for anti-BTLA antibody

Flow Cytometry (FC)

Other aplications not tested.

Application Notes for anti-BTLA antibody

Suitable for use in ELISA and Flow Cytometry.
Dilution: ELISA: Detection
Flow Cytometry: 1:50
Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml, after which they were washed and incubated 45 minutes on ice with 80ul of B5001-61K at a 1:50 dilution (5ug/ml).
2. They were then washed three times, fixed and analyzed by FACS using a lymphoid gate.
3. Cells stained positive with a mean shift of 0.54 log10 fluorescent units when compared to a Mouse IgG1 (FITC) negative control at a similar concentration.
4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml.

Other Notes

Small volumes of anti-BTLA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTLA purchase

MBS633646 is an antibody targeting BTLA. Also known as BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BTLA. It may not necessarily be applicable to this product.

NCBI GI #

25140977

NCBI Accession #

AAN71743.1

NCBI Official Full Name

BtlA

Protein Family

B- and T-lymphocyte attenuator

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.