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Hcd70-Mucd8 Fusion Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS634480

Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral… More details

$940 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
hCD70-muCD8 Fusion Protein
Full Product Name
hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)
Host
CHO cells
Form/Format
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Binds to cell surface CD27 on a subset of T cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight
34.8kD
Molecular Structure
 
A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD. Extracellular mature murine CD8alpha: (28)KPQAPEL....GLDFACD(194); Linking amino acids: GT; extracellular human CD70: (48)LPLESL....VQWVRP(193).

Product Categories/Family for hCD70-muCD8 Fusion Protein protein

Molecular Biology; MB-CD Markers
Applications Tested/Suitable for hCD70-muCD8 Fusion Protein protein
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes for hCD70-muCD8 Fusion Protein protein
Suitable for use in ELISA and Flow Cytometry.
Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells.
Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding).
2. They were incubated for 45 minutes on ice with 80ul of C2425-01QX at 10ug/ml. 3. Cells were washed twice and incubated with C2259-11D, after which they were washed three times, fixed and analyzed by FACS.
4. A net 57% sub population of the cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a buffer control. Binding was 99% blocked when reagent was first pre incubated with an excess of CD-70 antibody.

Testing Data of hCD70-muCD8 Fusion Protein protein

Binding of C2425-01QX to CD27+ human PBMC.
Protein – Cat# MBS634480

Other Notes

Small volumes of hCD70-muCD8 Fusion Protein protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hCD70-muCD8 Fusion Protein purchase

MBS634480 is a protein targeting hCD70-muCD8 Fusion Protein. Also known as hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein). Host: CHO cells.
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