Sepharose, Metal-chelating Reagent – Bioseparation

Metal-chelating Sepharose consists of iminodiacetic acid groups coupled to Sepharose. It is supplied free of metal ions and has to be charged with a suitable metal ion before use. It is not always possible to predict which metal ion is most appropriate. The metal ions most frequently used are Ni2+, Cu2+, Zn2+. A single exposed histidine residue may result in adsorption of the protein on Cu2+ while two vicinal histidine residues are needed for adsorption on Zn2+, Ni2+ ions are often used for the purification of polyhistidine-tagged fusion protein. In some cases Cu2+, Co2+, and Ca2+ have been used with success.

Small volumes of Sepharose, Metal-chelating reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS635427 is a reagent targeting Sepharose, Metal-chelating.