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Vimentin (VIM) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635654

Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached… More details

$680 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Recombinant Protein
Full Product Name
Vimentin, Recombinant, Human (FLJ36605, VIM)
Product Gene Name
VIM recombinant protein
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM EDTA, 2mM DTT, 10mM methylammonium chloride. Reconstitute with sterile ddH2O., 0.1% HSA or BSA to 0.1mg/ml. This solution can then be diluted into other aqueous solutions.
Purity/Purification
Purified
~ 95%; Determined by SDS-gel electrophoresis.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
57kD
Chromosome Location
Chromosome: 10; NC_000010.10 (17269934..17279592). Location: 10p13
OMIM
193060
3D Structure
ModBase 3D Structure for P08670

Product Categories/Family for VIM recombinant protein

Molecular Biology; MB-Extracellular Matrix Proteins
Applications Tested/Suitable for VIM recombinant protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes for VIM recombinant protein
Suitable for use in ELISA, Western Blot and Inhibition Studies.

Structure of VIM recombinant protein

Precursor- Protein structure and amino acid sequence:
Human VIM Recombinant Protein – Cat# MBS635654

Other Notes

Small volumes of VIM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS635654 is a recombinant protein targeting VIM. Also known as Vimentin, Recombinant, Human (FLJ36605, VIM). Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.3
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
CTRCT30
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
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Research Articles on VIM

1. vimentin expression was sufficient to induce increased mesenchymal/pro-metastasic phenotypic changes in melanoma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with VIM recombinant protein

 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with VIM recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >387 publications with VIM and Nervous System Diseases
 Neoplasms Antibodies  >291 publications with VIM and Neoplasms
 Brain Diseases Antibodies  >248 publications with VIM and Brain Diseases
 Neurodegenerative Diseases Antibodies  >168 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >47 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIM and Kidney Diseases
 Disease Models, Animal Antibodies  >34 publications with VIM and Disease Models, Animal
 Hemorrhage Antibodies  >33 publications with VIM and Hemorrhage
 Pain Antibodies  >31 publications with VIM and Pain
 Liver Diseases Antibodies  >29 publications with VIM and Liver Diseases

Organs/Tissues associated with VIM recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >473 publications with VIM and Brain
 Blood Antibodies  >192 publications with VIM and Blood
 Muscle Antibodies  >104 publications with VIM and Muscle
 Nerve Antibodies  >79 publications with VIM and Nerve
 Bone Antibodies  >69 publications with VIM and Bone
 Kidney Antibodies  >53 publications with VIM and Kidney
 Liver Antibodies  >51 publications with VIM and Liver
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Eye Antibodies  >40 publications with VIM and Eye
 Vascular Antibodies  >35 publications with VIM and Vascular
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