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Reverse Transcriptase, M-MLV Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636119

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (> 5kb). M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the… More details

$785 / 50K Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Reverse Transcriptase, M-MLV, Recombinant Protein
Full Product Name
Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
Host
Recombinant expressed in E Coli
Form/Format
Supplied as a liquid in 20mM Tris HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 0.01% NP-40 and 50% glycerol.
Purity/Purification
Molecular Biology Grade
90%, Verified by SDS-PAGE
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
71kD
CAS Number
9068-38-6
Unit Definition
One unit is defined as that amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37 degree C. The reaction conditions are: 50mM Tris-HCl (pH 8.3), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H] dTTP, 0.025mM oligo(dT50), 0.25mM poly(A)400 and 0.01% NP-40.

Product Categories/Family for Reverse Transcriptase, M-MLV recombinant protein

Molecular Biology; MB-Enzymes
Application Notes for Reverse Transcriptase, M-MLV recombinant protein
Suitable for use in In Situ Transcription, cDNA Library Synthesis and Screening, mRNA Structure Determination and Ribosomal Pause Site Determination.
Dilution: M-MLV Reverse Transcriptase is less processive than AMV Reverse Transcriptase, and therefore, more units of the M-MLV enzyme are required to generate the same amount of cDNA as in the AMV reaction. Thus, starting with 1µg of mRNA in a first-strand cDNA synthesis, 200 units of the M-MLV enzyme are recommended as opposed to 25 units of the AMV enzyme.
Optimal dilutions to be determined by researcher.

Other Notes

Small volumes of Reverse Transcriptase, M-MLV recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Reverse Transcriptase, M-MLV purchase

MBS636119 is a recombinant protein targeting Reverse Transcriptase, M-MLV. Also known as Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant. Host: Recombinant expressed in E Coli.
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