Hepatitis A Virus, VP1 (HAV) Recombinant Protein

Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins.

Recombinant protein corresponding to aa502-605 from HAV VP1 immunodominant regions, purified by proprietary chromatographic techniques, expressed in E. coli.

1. Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design. Haro I, Perez S, Chan WC, FEBS Lett 2003 Apr 10;540(1-3):133-40 2. Molecular evolution of hepatitis A virus: a new classification based on the complete VP1 protein. Costa-Mattioli M, Cristina J, Perez-Bercof R, Vega I, J Virol 2002 Sep;76(18):9516-25 3. Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C. Emerson SU, Huang YK, Brockington A, Purcell RH, J Virol 2002 Sep;76(17):8551-9 4. A proposed vestigial translation initiation motif in VP1 of hepatitis A virus. Kang JA, Funkhouser AW, Virus Res 2002 Jul;87(1):11-9 5. Maturation of the hepatitis A virus capsid protein VP1 is not dependent on processing by the 3Cpro proteinase. Martin A, Benichou D, Cohen LM, J Virol 1999 Aug;73(8):6220-7 6.

Small volumes of HAV recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS6508522 is a recombinant protein targeting HAV. Also known as Hepatitis A Virus, VP1 Recombinant, aa502-605 (HAV). Host: E Coli.