MTHFD2 Recombinant Protein – Cat# MBS637342

SDS: Analysis of MTHFD2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining

Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2) Recombinant Protein

Name: MTHFD2 Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS637342
Price: 590 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637342

This protein is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for… More details

$590 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2), Recombinant Protein

Full Product Name

Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2, NAD+ Dependent Methylene Tetrahydrofolate Dehydrogenase Cyclohydrolase) Recombinant, Human

Product Gene Name

MTHFD2 recombinant protein

Host

E Coli; full length (aa1-344)

Form/Format

Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% sodium azide.

Purity/Purification

Highly Purified
> 95%

Preparation and Storage

For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6-12 months at -70 degree C.

Molecular Weight

37,895 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (74425690..74442425). Location: 2p13.1

OMIM

604887

3D Structure

ModBase 3D Structure for P13995

Tag Fusion Partner

T7 Tag at N-terminus

Quality Control

SDS-PAGE; Important Note: This recombinant enzyme has NOT been tested with regard to biological activity.

Product Categories/Family for MTHFD2 recombinant protein

Molecular Biology; MB-Enzymes; Dehydrogenase

Applications Tested/Suitable for MTHFD2 recombinant protein

ELISA (EL/EIA), Western Blot (WB)

Application Notes for MTHFD2 recombinant protein

Suitable for use as an antigen, ligand, or assay standard.

Other Notes

Small volumes of MTHFD2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MTHFD2 purchase

MBS637342 is a recombinant protein targeting MTHFD2. Also known as Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2, NAD+ Dependent Methylene Tetrahydrofolate Dehydrogenase Cyclohydrolase) Recombinant, Human. Host: E Coli; full length (aa1-344).

Additional Details

NCBI/Uniprot data below describe general gene information for MTHFD2. It may not necessarily be applicable to this product.

NCBI GI #

62897643

NCBI GeneID

10797

NCBI Accession #

BAD96761.1

UniProt Primary Accession #

P13995

UniProt Secondary Accession #

Q53G90; Q53GV5; Q53S36

UniProt Related Accession #

P13995

NCBI Official Full Name

methylene tetrahydrofolate dehydrogenase 2 variant

NCBI Official Synonym Full Names

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase

NCBI Official Symbol

MTHFD2

NCBI Official Synonym Symbols

NMDMC

UniProt Protein Name

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Protein Family

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase

UniProt Gene Name

MTHFD2

UniProt Synonym Gene Names

NMDMC

UniProt Entry Name

MTDC_HUMAN

NCBI Protein Information

bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase

NCBI Summary for MTHFD2

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

UniProt Comments for MTHFD2

MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Hydrolase; EC 1.5.1.15; EC 3.5.4.9; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; mitochondrion; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; magnesium ion binding; methylenetetrahydrofolate dehydrogenase (NAD+) activity; formate-tetrahydrofolate ligase activity; phosphate binding; methenyltetrahydrofolate cyclohydrolase activity

Biological Process: tetrahydrofolate metabolic process; folic acid and derivative biosynthetic process; one-carbon compound metabolic process

Research Articles on MTHFD2

1. Data indicate that the reduced vimentin expression in response to EPHB4, WIPF2 and MTHFD2 silencing was observed at mRNA and protein levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MTHFD2 recombinant protein

Reference Product	PubMed Publications
MTHFR recombinant protein	>4 publications with MTHFD2 and MTHFR
MTHFD1L recombinant protein	>3 publications with MTHFD2 and MTHFD1L
TYMS recombinant protein	>1 publications with MTHFD2 and TYMS
SHMT1 recombinant protein	>1 publications with MTHFD2 and SHMT1

Pathways associated with MTHFD2 recombinant protein

Products by Pathway	Pathway Diagram
C1-unit Interconversion, Eukaryotes Pathway antibodies	C1-unit Interconversion, Eukaryotes Pathway Diagram
C1-unit Interconversion, Eukaryotes Pathway antibodies	C1-unit Interconversion, Eukaryotes Pathway Diagram
Nucleotide Metabolism Pathway antibodies	Nucleotide Metabolism Pathway Diagram
One Carbon Metabolism Pathway antibodies	One Carbon Metabolism Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram
Folate Transformations I Pathway antibodies	Folate Transformations I Pathway Diagram
Histidine Degradation III Pathway antibodies	Histidine Degradation III Pathway Diagram
Tetrahydrofolate Salvage From 5,10-methenyltetrahydrofolate Pathway antibodies	Tetrahydrofolate Salvage From 5,10-methenyltetrahydrofolate Pathway Diagram

Diseases associated with MTHFD2 recombinant protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>7 publications with MTHFD2 and Neoplasms
Breast Neoplasms Antibodies	>4 publications with MTHFD2 and Breast Neoplasms
Nervous System Diseases Antibodies	>3 publications with MTHFD2 and Nervous System Diseases
Neural Tube Defects Antibodies	>2 publications with MTHFD2 and Neural Tube Defects
Urinary Bladder Neoplasms Antibodies	>1 publications with MTHFD2 and Urinary Bladder Neoplasms
Adenocarcinoma Antibodies	>1 publications with MTHFD2 and Adenocarcinoma
Cardiovascular Diseases Antibodies	>1 publications with MTHFD2 and Cardiovascular Diseases
Brain Diseases Antibodies	>1 publications with MTHFD2 and Brain Diseases
Liver Diseases Antibodies	>1 publications with MTHFD2 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with MTHFD2 and Carcinoma, Hepatocellular

Organs/Tissues associated with MTHFD2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2 publications with MTHFD2 and Liver
Embryonic Tissue Antibodies	>2 publications with MTHFD2 and Embryonic Tissue
Nerve Antibodies	>1 publications with MTHFD2 and Nerve
Muscle Antibodies	>1 publications with MTHFD2 and Muscle
Heart Antibodies	>1 publications with MTHFD2 and Heart
Bone Antibodies	>1 publications with MTHFD2 and Bone
Bladder Antibodies	>1 publications with MTHFD2 and Bladder
Brain Antibodies	>1 publications with MTHFD2 and Brain