Ficolin 1 (FCN1) Human Active Protein

Mouse myeloma NSO cell line-derived recombinant human Ficolin-1 (Gln28 thru Ala326, NCBI accession #O00602) with a C-terminal 10x His tag.

Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1-3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2-5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4-6). The 35kD, 326 amino acid (aa) human ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like ficolin-2, larger homo-multimers of ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4-7). Like other ficolins, ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4-6). The ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).

1. Endo, Y. et al., 2006, Adv. Exp. Med. Biol. 586:265.
2. Endo, Y. et al., 1996, Genomics 36:515. Scand. J. Immunol. 62:462.
3. Lu, J. et al., 1996, Biochem. J. 313: 473.
4. Liu, Y. et al., 2005, J. Immunol. 175:3150.
5. . Teh, C. et al., 2000, Immunology 101:225.
6. Frederiksen, P. D. et al., 2005,
7. Tanio, M. et al., 2007, J. Biol. Chem. 282:3889.
8. Endo, Y. et al., 2004, Genomics 84:737.

Small volumes of FCN1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS638134 is an active protein targeting FCN1. Also known as Ficolin 1, Recombinant, Human (Ficolin-M). Reactive in Human. Host: Recombinant, Human CD33 Signal Peptide.