Complement Factor H-Related Protein 5 (CFHR5) Active Protein

Name: CFHR5 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS638289
Price: 1215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638289

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1(FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Complement Factor H-Related Protein 5 (CFHR5), Active Protein

Full Product Name

Complement Factor H-Related Protein 5, Recombinant, Human (FHR5)

Product Gene Name

CFHR5 active protein

Form/Format

Supplied as a lyophilized powder in 25mM Tris, 0.4M sodium chloride, pH 7.5.

Purity/Purification

Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.

Preparation and Storage

Samples are stable for up to twelve months at -20 degree C in a manual defrost freezer after the date of receipt. After reconstitution, this protein can be aliquoted and stored at above temperature for up to three months without a significant loss of activity. Avoid repeated freeze-thaw cycles.

Molecular Weight

The purified, secreted rhCFHR5 has a major N-terminal sequence of E19GTLCDFPKI. The 561 amino acid residue rhCFHR5 predicts a molecular mass of 64 kD and migrates with an apparent molecular mass of approximately 73kD in SDS-PAGE under reducing conditions.

Chromosome Location

Chromosome: 1; NC_000001.10 (196946667..196978804). Location: 1q31.3

OMIM

608593

3D Structure

ModBase 3D Structure for Q9BXR6

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

Measured by its ability to inhibit the binding of biotinylated rhCFHR5 to immobilized rhCRP (McRae, JL., et al., 2005, J. Immunol. 174:6250).

Endotoxin Level

< 1.0 EU per 1 g of the protein as determined by the LAL method.

Reconstitution

It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Product Categories/Family for CFHR5 active protein

Molecular Biology; MB-Complement

Other Notes

Small volumes of CFHR5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFHR5 purchase

MBS638289 is an active protein targeting CFHR5. Also known as Complement Factor H-Related Protein 5, Recombinant, Human (FHR5).

Additional Details

NCBI/Uniprot data below describe general gene information for CFHR5. It may not necessarily be applicable to this product.

NCBI GI #

13540563

NCBI GeneID

81494

NCBI Accession #

NP_110414.1

UniProt Primary Accession #

Q9BXR6

UniProt Secondary Accession #

Q2NKK2

UniProt Related Accession #

Q9BXR6

GenBank Nucleotide #

NM_030787.3

NCBI Official Full Name

complement factor H-related protein 5

NCBI Official Synonym Full Names

complement factor H-related 5

NCBI Official Symbol

CFHR5

NCBI Official Synonym Symbols

FHR5; CFHL5; FHR-5; CFHR5D

UniProt Protein Name

Complement factor H-related protein 5

Protein Family

Complement factor H-related protein

UniProt Gene Name

CFHR5

UniProt Synonym Gene Names

CFHL5; FHR5; FHR-5

UniProt Entry Name

FHR5_HUMAN

NCBI Protein Information

complement factor H-related protein 5

NCBI Summary for CFHR5

This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq, Jan 2010]

UniProt Comments for CFHR5

CFHR5: Involved in complement regulation. Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: extracellular region

Biological Process: complement activation, alternative pathway

Disease: Cfhr5 Deficiency

Research Articles on CFHR5

1. Recent investigations in London and Cyprus culminated in the identification of another autosomal dominant condition that presents with microscopic haematuria because of heterozygous mutations in the CFHR5 gene--{review}

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CFHR5 active protein

Reference Product	PubMed Publications
CFI active protein	>4 publications with CFHR5 and CFI

Diseases associated with CFHR5 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>5 publications with CFHR5 and Inflammation
Atrophy Antibodies	>1 publications with CFHR5 and Atrophy