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Tati Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638367

MBS638367 is a restriction enzyme targeting TatI. 5'-(A/T)^G T A C (A/T)-3' More details

$515 / 100 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TatI, Restriction Enzyme
Full Product Name
TatI
Form/Format
Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Compatible Ends
Acc65I, BshNI, Bsp1407I, Pfl23II
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
24
Ligation/Recutting Assay
After 2-fold overdigestion (2u/ug DNA x 1 hour) with TatI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.2uM. More than 95% of these can be recut.
M13mp18/19
5
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 5-fold overdigestion (5u/ug lambda DNA x 1 hour) with TatI (see Star Activity).
pBR322
2
PhiX174
0
pTZ19R/U
1
pUC18/19
2
pUC57
2
Stability During Prolonged Incubation
A minimum of 0.2 units of enzymes is required for complete digestion of 1ug of lambda DNA in 16 hours at 65 degree C.
Thermal Inactivation
Enzyme is not inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 65 degree C in 50ul of assay buffer.

Product Categories/Family for TatI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of TatI restriction enzyme

Restriction Enzyme – Cat# MBS638367

Other Notes

Small volumes of TatI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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