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Eco91i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638446

MBS638446 is a restriction enzyme targeting Eco91I. Also known as Eco91I (BstEII). Host: Escherichia coli RFL91. 5'-G↓G T N A C C-3'3'-C C A N T G↑G-5' More details

$435 / 1000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Eco91I, Restriction Enzyme
Full Product Name
Eco91I (BstEII)
Host
Escherichia coli RFL91
Purity/Purification
Molecular Biology Grade
Concentration
10u/ul (lot specific)
Preparation and Storage
Aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied With
- Restriction Enzyme Buffer A: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
- Restriction Enzyme Buffer D: 10X: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA when diluted to 1X. Incubate at 37°C.
Unit Definition
One unit is defined as the amount of Eco91I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.
Diluent Buffer
10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer
10mM Tris-HCl, pH 7.5, 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Enzyme Properties
Methylation Effects:
Dam: never overlaps - no effect.
Dcm/CpG: may overlap - no effect.
EcoKl/EcoBl: may overlap - effect not determined.

Stability during Prolonged Incubation:
A minimum of 0.1 units of MBS638446 is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.

Thermal Inactivation:
MBS638446 is inactivated by incubation at 65°C for 20 minutes.

Digestion of Agarose-embedded DNA:
A minimum of 5 units of MBS638446 is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Compatible Ends:
G↓GT(C/G)ACC–MaeIII, NmuCI
G↓GT(A/T)ACC–MaeIII

Number of Recognition Sites in DNA:
Lambda: 13
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 0
pTZ19R/U: 0
Recommended Protocol for Digestion
Add:
Nuclease-free water: 16ul
Restriction Enzyme Buffer D: 2ul
DNA (0.5-1ug/ul): 1ul
Eco91I: 0.5-2ul*

Mix gently and spin down for a few seconds. Incubate at 37°C for 1-16 hours*. The digestion reaction may be scaled either up or down.

Product Categories/Family for Eco91I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction
Application Notes for Eco91I restriction enzyme
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion with Eco911 (10u/ug lambda DNA x 16 hours).

Ligation/Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Eco911for 4 hours.

Blue/White Cloning Assay: The B/W assay was replaced by the L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that equals to that of B/W test.

Testing Data of Eco91I restriction enzyme

Restriction Enzyme – Cat# MBS638446

Other Notes

Small volumes of Eco91I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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