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AHSG Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641354

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AHSG, Polyclonal Antibody
Full Product Name
AHSG, CT (AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B)
Product Gene Name
anti-AHSG antibody
Product Synonym Names
Anti -AHSG, CT (AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
39,325 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (186330850..186339107). Location: 3q27
OMIM
138680
3D Structure
ModBase 3D Structure for P02765
Immunogen
AHSG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-284 amino acids from the C-terminal region of human AHSG.

Product Categories/Family for anti-AHSG antibody

Antibodies; Abs to Carbohydrates; Glycoproteins
Applications Tested/Suitable for anti-AHSG antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-AHSG antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunofluorescence 1:10-50

Western Blot (WB) of anti-AHSG antibody

Western Blot analysis in human plasma lysate (20ug/lane) using MBS641354 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 39kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-AHSG Polyclonal Antibody – Cat# MBS641354

Western Blot (WB) of anti-AHSG antibody

Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS641354. This demonstrates that MBS641354 detected the AHSG protein (arrow).
Rabbit Anti-AHSG Polyclonal Antibody, Western Blot (WB) – Cat# MBS641354

Immunohistochemistry (IHC) of anti-AHSG antibody

Immunohistochemistry (FFPE) analysis in human liver tissue using MBS641354 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Rabbit Anti-AHSG Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS641354

Immunofluorescence (IF) of anti-AHSG antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with 1:25 (1:25), followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody (1:200) (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Rabbit Anti-AHSG Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS641354

Flow Cytometry (FC/FACS) of anti-AHSG antibody

Flow Cytometric analysis of HepG2 cells using MBS641354 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AHSG Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS641354

Other Notes

Small volumes of anti-AHSG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AHSG purchase

MBS641354 is an antibody targeting AHSG. Also known as AHSG, CT (AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AHSG. It may not necessarily be applicable to this product.

NCBI GI #
156523970
NCBI GeneID
197
NCBI Accession #
NP_001613.2
UniProt Primary Accession #
P02765
UniProt Secondary Accession #
O14961; O14962; Q9P152; A8K9N6; B2R7G1
UniProt Related Accession #
P02765; AAB29984
GenBank Nucleotide #
NM_001622.2
NCBI Official Full Name
alpha-2-HS-glycoprotein preproprotein
NCBI Official Synonym Full Names
alpha-2-HS-glycoprotein
NCBI Official Symbol
AHSG
NCBI Official Synonym Symbols
AHS; A2HS; HSGA; FETUA
UniProt Protein Name
Alpha-2-HS-glycoprotein
UniProt Synonym Protein Names
Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-A
Protein Family
Alpha-2-HS-glycoprotein
UniProt Gene Name
AHSG
UniProt Synonym Gene Names
FETUA
UniProt Entry Name
FETUA_HUMAN
NCBI Protein Information
alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein

UniProt Comments for AHSG

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region

Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity

Biological Process: pinocytosis; negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; regulation of bone mineralization; skeletal development
Show more

Research Articles on AHSG

1. Report serum fetuin-A concentrations acorss the spectrum of chronic kidney disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AHSG antibody

 Reference Product  PubMed Publications
 BMP2 antibody  >2 publications with AHSG and BMP2
 ALB antibody  >2 publications with AHSG and ALB
 AMBP antibody  >2 publications with AHSG and AMBP
 BMP4 antibody  >1 publications with AHSG and BMP4
 APOA2 antibody  >1 publications with AHSG and APOA2
 APOH antibody  >1 publications with AHSG and APOH

Pathways associated with anti-AHSG antibody

 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram

Diseases associated with anti-AHSG antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >133 publications with AHSG and Cardiovascular Diseases
 Kidney Diseases Antibodies  >105 publications with AHSG and Kidney Diseases
 Calcinosis Antibodies  >94 publications with AHSG and Calcinosis
 Inflammation Antibodies  >78 publications with AHSG and Inflammation
 Neoplasms Antibodies  >54 publications with AHSG and Neoplasms
 Heart Diseases Antibodies  >52 publications with AHSG and Heart Diseases
 Liver Diseases Antibodies  >36 publications with AHSG and Liver Diseases
 Brain Diseases Antibodies  >19 publications with AHSG and Brain Diseases
 Liver Neoplasms Antibodies  >17 publications with AHSG and Liver Neoplasms
 Hypertension Antibodies  >17 publications with AHSG and Hypertension

Organs/Tissues associated with anti-AHSG antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >471 publications with AHSG and Blood
 Liver Antibodies  >80 publications with AHSG and Liver
 Heart Antibodies  >37 publications with AHSG and Heart
 Muscle Antibodies  >33 publications with AHSG and Muscle
 Brain Antibodies  >19 publications with AHSG and Brain
 Lung Antibodies  >10 publications with AHSG and Lung
 Embryonic Tissue Antibodies  >4 publications with AHSG and Embryonic Tissue
 Spleen Antibodies  >3 publications with AHSG and Spleen
 Thymus Antibodies  >2 publications with AHSG and Thymus
 Testis Antibodies  >2 publications with AHSG and Testis
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