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AKT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641825

AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors.… More details

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Product Overview

Online Datasheet in PDF
Product Name
AKT2, Polyclonal Antibody
Full Product Name
AKT2, CT (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta)
Product Gene Name
anti-AKT2 antibody
Product Synonym Names
Anti -AKT2, CT (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human AKT2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
55,769 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (40736224..40791443, complement). Location: 19q13.1-q13.2
OMIM
125853
3D Structure
ModBase 3D Structure for P31751
Immunogen
Synthetic peptide selected from the C-terminal region of human AKT2 (KLH).

Product Categories/Family for anti-AKT2 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-AKT2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AKT2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100

Immunofluorescence (IF) of anti-AKT2 antibody

Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (C-term).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).AKT2 immunoreactivity is localized to Cytoplasm significantly.
Rabbit Anti-AKT2 Polyclonal Antibody – Cat# MBS641825

Western Blot (WB) of anti-AKT2 antibody

Western blot analysis of AKT2 (arrow) using AKT2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
Rabbit Anti-AKT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS641825

Immunohistochemistry (IHC) of anti-AKT2 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-AKT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS641825

Flow Cytometry (FC/FACS) of anti-AKT2 antibody

AKT2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AKT2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS641825

Other Notes

Small volumes of anti-AKT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKT2 purchase

MBS641825 is an antibody targeting AKT2. Also known as AKT2, CT (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT2. It may not necessarily be applicable to this product.

NCBI GI #
111309392
NCBI GeneID
208
NCBI Accession #
AAI20996.1
UniProt Primary Accession #
P31751
UniProt Secondary Accession #
Q0VAN1; Q68GC0; B2RBD8
UniProt Related Accession #
P31751
NCBI Official Full Name
AKT2 protein
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
UniProt Protein Name
RAC-beta serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
Protein Family
Potassium channel
UniProt Gene Name
AKT2
UniProt Synonym Gene Names
PKB beta
UniProt Entry Name
AKT2_HUMAN
NCBI Protein Information
RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; protein kinase Akt-2; protein kinase B beta; rac protein kinase beta; murine thymoma viral (v-akt) homolog-2

NCBI Summary for AKT2

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]
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UniProt Comments for AKT2

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; plasma membrane; cell cortex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding

Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; carbohydrate transport; positive regulation of glycogen biosynthetic process; myelin maintenance in the peripheral nervous system; intracellular protein transport across a membrane; protein modification process; positive regulation of vesicle fusion; glucose metabolic process; signal transduction; regulation of cell migration; protein amino acid phosphorylation; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation

Disease: Hypoinsulinemic Hypoglycemia With Hemihypertrophy; Diabetes Mellitus, Noninsulin-dependent
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Research Articles on AKT2

1. Akt2 regulates metastatic potential in neuroblastoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AKT2 antibody

 Reference Product  PubMed Publications
 MTOR antibody  >77 publications with AKT2 and MTOR
 FOXO1 antibody  >26 publications with AKT2 and FOXO1
 TRIB3 antibody  >2 publications with AKT2 and TRIB3
 GSK3B antibody  >2 publications with AKT2 and GSK3B
 AKT1S1 antibody  >2 publications with AKT2 and AKT1S1
 INS antibody  >2 publications with AKT2 and INS
 CHUK antibody  >1 publications with AKT2 and CHUK
 TCL1A antibody  >1 publications with AKT2 and TCL1A

Pathways associated with anti-AKT2 antibody

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PKB Pathway antibodies  Activation Of PKB Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram

Diseases associated with anti-AKT2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >314 publications with AKT2 and Neoplasms
 Breast Neoplasms Antibodies  >68 publications with AKT2 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >63 publications with AKT2 and Ovarian Neoplasms
 Adenocarcinoma Antibodies  >58 publications with AKT2 and Adenocarcinoma
 Lung Neoplasms Antibodies  >33 publications with AKT2 and Lung Neoplasms
 Lung Diseases Antibodies  >30 publications with AKT2 and Lung Diseases
 Neoplasms, Experimental Antibodies  >30 publications with AKT2 and Neoplasms, Experimental
 Liver Diseases Antibodies  >29 publications with AKT2 and Liver Diseases
 Pancreatic Neoplasms Antibodies  >29 publications with AKT2 and Pancreatic Neoplasms
 Nervous System Diseases Antibodies  >27 publications with AKT2 and Nervous System Diseases

Organs/Tissues associated with anti-AKT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >157 publications with AKT2 and Muscle
 Blood Antibodies  >105 publications with AKT2 and Blood
 Liver Antibodies  >85 publications with AKT2 and Liver
 Lung Antibodies  >48 publications with AKT2 and Lung
 Vascular Antibodies  >45 publications with AKT2 and Vascular
 Adipose Tissue Antibodies  >41 publications with AKT2 and Adipose Tissue
 Brain Antibodies  >41 publications with AKT2 and Brain
 Heart Antibodies  >32 publications with AKT2 and Heart
 Bone Antibodies  >29 publications with AKT2 and Bone
 Connective Tissue Antibodies  >27 publications with AKT2 and Connective Tissue
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