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ZNF473 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642003

MBS642003 is an antibody targeting ZNF473. Also known as ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog). Reactive in Human. Host: Rabbit. More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZN473 (ZNF473), Polyclonal Antibody
Full Product Name
ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog)
Product Gene Name
Product Synonym Names
Anti -ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
100,182 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (50528786..50552033). Location: 19q13.33
3D Structure
ModBase 3D Structure for Q8WTR7
Immunogen
ZN473 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185~215 amino acids from the N-terminal region of human ZN473.
Applications Tested/Suitable for anti-ZNF473 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ZNF473 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ZNF473 antibody

Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.
Rabbit Anti-ZNF473 Polyclonal Antibody – Cat# MBS642003

Flow Cytometry (FC/FACS) of anti-ZNF473 antibody

ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ZNF473 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS642003

Other Notes

Small volumes of anti-ZNF473 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZNF473. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein 473
NCBI Official Synonym Full Names
zinc finger protein 473
NCBI Official Symbol
ZNF473
NCBI Official Synonym Symbols
ZN473; HZFP100
UniProt Protein Name
Zinc finger protein 473
UniProt Synonym Protein Names
Zinc finger protein 100 homolog
Protein Family
UniProt Gene Name
ZNF473
UniProt Synonym Gene Names
KIAA1141; ZFP100; Zfp-100
UniProt Entry Name
ZN473_HUMAN
NCBI Protein Information
zinc finger protein 473; zfp-100; zinc finger protein ZFP100; zinc finger protein 100 homolog

UniProt Comments for ZNF473

ZNF473: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; Cajal body

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; histone mRNA metabolic process; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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