Confocal immunofluorescent analysis of HeLa cells using Di/Tri-Methyl-Histone H3 (Lys9) Mouse mAb #MBS642110 (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 HeLa cells and either 10ul of Di/Tri-Methyl-Histone H3 (Lys9) Mouse mAb #MBS642110 or 2ul Normal Rabbit IgG, using Enzymatic Chromatin IP Kit (Magnetic Beads). The enriched DNA was quantified by real-time PCR using Human GAPDH Exon 1 Primers, Human RPL30 Exon 3 Primers, Human α Satellite Repeat Primers, and Human AFM Intron 1 Primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.