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RASGRP3 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642166

MBS642166 is an antibody targeting RASGRP3. Also known as RASGRP3 (Ras Guanyl-releasing Protein 3, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor III, GRP3, Guanine Nucleotide Exchange Factor for Rap1, KIAA0846). Reactive in Human. Host: Mouse. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RASGRP3, Polyclonal Antibody
Full Product Name
RASGRP3 (Ras Guanyl-releasing Protein 3, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor III, GRP3, Guanine Nucleotide Exchange Factor for Rap1, KIAA0846)
Product Gene Name
Product Synonym Names
Anti -RASGRP3 (Ras Guanyl-releasing Protein 3, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor III, GRP3, Guanine Nucleotide Exchange Factor for Rap1, KIAA0846)
Immunogen Sequence
MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV HAGVDVVDRG TEFELDQDEG EETRQDGEDG
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RASGRP3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
78,332 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (33661416..33789798). Location: 2p25.1-p24.1
OMIM
609531
3D Structure
ModBase 3D Structure for Q8IV61
Immunogen
Full length human RASGRP3, aa1-690 (NP_733772.1).
Applications Tested/Suitable for anti-RASGRP3 antibody
Western Blot (WB)
Application Notes for anti-RASGRP3 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-RASGRP3 antibody

RASGRP3 polyclonal antibody. Western Blot analysis of RASGRP3 expression in human placenta.
Mouse Anti-RASGRP3 Polyclonal Antibody – Cat# MBS642166

Western Blot (WB) of anti-RASGRP3 antibody

Western Blot analysis of RASGRP3 expression in transfected 293T cell line by RASGRP3 polyclonal antibody. Lane 1: RASGRP3 transfected lysate (75.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-RASGRP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS642166

Other Notes

Small volumes of anti-RASGRP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RASGRP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras guanyl-releasing protein 3 isoform 1
NCBI Official Synonym Full Names
RAS guanyl releasing protein 3 (calcium and DAG-regulated)
NCBI Official Symbol
RASGRP3
NCBI Official Synonym Symbols
GRP3
UniProt Protein Name
Ras guanyl-releasing protein 3
UniProt Synonym Protein Names
Calcium and DAG-regulated guanine nucleotide exchange factor III; Guanine nucleotide exchange factor for Rap1
UniProt Gene Name
RASGRP3
UniProt Synonym Gene Names
GRP3; KIAA0846
UniProt Entry Name
GRP3_HUMAN
NCBI Protein Information
ras guanyl-releasing protein 3; guanine nucleotide exchange factor for Rap1; calcium and DAG-regulated guanine nucleotide exchange factor III

NCBI Summary for RASGRP3

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
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UniProt Comments for RASGRP3

RASGRP3: Guanine nucleotide exchange factor (GEF) for Ras and Rap1. Belongs to the RASGRP family.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 2p25.1-p24.1

Cellular Component: integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; signal transducer activity; Ras GTPase binding; guanyl-nucleotide exchange factor activity; calcium ion binding; kinase binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: small GTPase mediated signal transduction; Ras protein signal transduction; MAPKKK cascade; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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