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ARSF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642450

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ARSF, Polyclonal Antibody
Full Product Name
ARSF, ID (ARSF, Arylsulfatase F)
Product Gene Name
anti-ARSF antibody
Product Synonym Names
Anti -ARSF, ID (ARSF, Arylsulfatase F)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
65,940 Da
Chromosome Location
Chromosome: X; NC_000023.10 (2958275..3030770). Location: Xp22.3
OMIM
300003
3D Structure
ModBase 3D Structure for P54793
Immunogen
ARSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the Central region of human ARSF.

Product Categories/Family for anti-ARSF antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-ARSF antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ARSF antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ARSF antibody

ARSF Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ARSF antibody detected the ARSF protein (arrow).
Rabbit Anti-ARSF Polyclonal Antibody – Cat# MBS642450

Western Blot (WB) of anti-ARSF antibody

Western blot analysis of ARSF (arrow) using rabbit polyclonal ARSF Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.
Rabbit Anti-ARSF Polyclonal Antibody, Western Blot (WB) – Cat# MBS642450

Flow Cytometry (FC/FACS) of anti-ARSF antibody

ARSF Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ARSF Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS642450

Other Notes

Small volumes of anti-ARSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARSF purchase

MBS642450 is an antibody targeting ARSF. Also known as ARSF, ID (ARSF, Arylsulfatase F). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARSF. It may not necessarily be applicable to this product.

NCBI GI #
31742482
NCBI GeneID
416
NCBI Accession #
NP_004033.2
UniProt Primary Accession #
P54793
UniProt Secondary Accession #
Q8TCC5
UniProt Related Accession #
P54793
GenBank Nucleotide #
NM_004042.4
NCBI Official Full Name
arylsulfatase F
NCBI Official Synonym Full Names
arylsulfatase F
NCBI Official Symbol
ARSF
NCBI Official Synonym Symbols
ASF
UniProt Protein Name
Arylsulfatase F
Protein Family
Arylsulfatase
UniProt Gene Name
ARSF
UniProt Synonym Gene Names
ASF
UniProt Entry Name
ARSF_HUMAN
NCBI Protein Information
arylsulfatase F

NCBI Summary for ARSF

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]
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UniProt Comments for ARSF

ARSF: a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]

Protein type: EC 3.1.6.-; Secreted, signal peptide; Hydrolase; Secreted

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: endoplasmic reticulum lumen

Molecular Function: arylsulfatase activity; metal ion binding

Biological Process: sphingolipid metabolic process; cellular protein metabolic process; glycosphingolipid metabolic process; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARSF antibody

 Reference Product  PubMed Publications
 ARSE antibody  >3 publications with ARSF and ARSE
 ARSD antibody  >2 publications with ARSF and ARSD
 ARSG antibody  >1 publications with ARSF and ARSG
 ARSA antibody  >1 publications with ARSF and ARSA

Pathways associated with anti-ARSF antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies  PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 The Activation Of Arylsulfatases Pathway antibodies  The Activation Of Arylsulfatases Pathway Diagram

Organs/Tissues associated with anti-ARSF antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with ARSF and Testis
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