Goat Anti-DEFA1 Polyclonal Antibody – Cat# MBS642872

Western Blot analysis of human spleen lysate (35ug protein in RIPA buffer) using MBS642872 (2ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Neutrophil Defensin 1, 3 (DEFA1) Antibody – Goat Polyclonal

Name: DEFA1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS642872
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642872

Defensin alpha 1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.Defensin 2 and defensin 3 have… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neutrophil Defensin 1, 3 (DEFA1), Polyclonal Antibody

Full Product Name

Neutrophil Defensin 1, 3 (Defensin, alpha 1, HNP-1, HP-1, HP1, DEFA1, DEF1, DEFA2, MRS, DEFA1B/Defensin, alpha 3, HNP-3, HP-3, HP3, DEFA3, DEF3)

Product Gene Name

anti-DEFA1 antibody

Product Synonym Names

Anti -Neutrophil Defensin 1, 3 (Defensin, alpha 1, HNP-1, HP-1, HP1, DEFA1, DEF1, DEFA2, MRS, DEFA1B/Defensin, alpha 3, HNP-3, HP-3, HP3, DEFA3, DEF3)

Clonality

Polyclonal

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human DEFA1,3.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

10,201 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (6835171..6837614, complement). Location: 8p23.1

OMIM

125220

3D Structure

ModBase 3D Structure for P59665

Immunogen

Synthetic peptide corresponding to C-DESLAPKHPGSRKN, from human DEFA1,3, at the internal region of the protein (NP_004075.1, NP_005208.1).

Product Categories/Family for anti-DEFA1 antibody

Antibodies; Abs to Defensin

Applications Tested/Suitable for anti-DEFA1 antibody

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-DEFA1 antibody

Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: ELISA: 1:128,000
Western Blot: 1-3ug/ml
Immunohistochemistry (Paraffin): 4-6ug/ml

Other Notes

Small volumes of anti-DEFA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFA1 purchase

MBS642872 is an antibody targeting DEFA1. Also known as Neutrophil Defensin 1, 3 (Defensin, alpha 1, HNP-1, HP-1, HP1, DEFA1, DEF1, DEFA2, MRS, DEFA1B/Defensin, alpha 3, HNP-3, HP-3, HP3, DEFA3, DEF3). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for DEFA1. It may not necessarily be applicable to this product.

NCBI GI #

4758146

NCBI GeneID

1667

NCBI Accession #

NP_004075.1

UniProt Primary Accession #

P59665

UniProt Secondary Accession #

P11479; Q14125; Q6EZF6

UniProt Related Accession #

P59665

GenBank Nucleotide #

NM_004084.3

NCBI Official Full Name

neutrophil defensin 1 preproprotein

NCBI Official Synonym Full Names

defensin, alpha 1

NCBI Official Symbol

DEFA1

NCBI Official Synonym Symbols

HP1; MRS; DEF1; HP-1; DEFA2; HNP-1

UniProt Protein Name

Neutrophil defensin 1

UniProt Synonym Protein Names

Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2

Protein Family

Alpha-defensin

UniProt Gene Name

DEFA1

UniProt Synonym Gene Names

DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

UniProt Entry Name

DEF1_HUMAN

NCBI Protein Information

neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence

NCBI Summary for DEFA1

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

UniProt Comments for DEFA1

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus

Research Articles on DEFA1

1. Results indicate that the gene expression of DEFA 1/3 and 4 was significantly increased in all tumours - except for a significant decrease of DEFA 4 gene expression in pleomorphic adenomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DEFA1 antibody

Reference Product	PubMed Publications
DEFA3 antibody	>9 publications with DEFA1 and DEFA3
DEFB1 antibody	>4 publications with DEFA1 and DEFB1
DEFB4A antibody	>1 publications with DEFA1 and DEFB4A

Pathways associated with anti-DEFA1 antibody

Products by Pathway	Pathway Diagram
Alpha-defensins Pathway antibodies	Alpha-defensins Pathway Diagram
Cellular Roles Of Anthrax Toxin Pathway antibodies	Cellular Roles Of Anthrax Toxin Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with anti-DEFA1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>8 publications with DEFA1 and Inflammation
Neoplasms Antibodies	>3 publications with DEFA1 and Neoplasms
Congenital Abnormalities Antibodies	>1 publications with DEFA1 and Congenital Abnormalities
Liver Diseases Antibodies	>1 publications with DEFA1 and Liver Diseases
Adenocarcinoma Antibodies	>1 publications with DEFA1 and Adenocarcinoma
Craniofacial Abnormalities Antibodies	>1 publications with DEFA1 and Craniofacial Abnormalities
Leukemia Antibodies	>1 publications with DEFA1 and Leukemia
Fibrosis Antibodies	>1 publications with DEFA1 and Fibrosis
Necrosis Antibodies	>1 publications with DEFA1 and Necrosis
Bone Marrow Diseases Antibodies	>1 publications with DEFA1 and Bone Marrow Diseases

Organs/Tissues associated with anti-DEFA1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>16 publications with DEFA1 and Blood
Bone Antibodies	>3 publications with DEFA1 and Bone
Liver Antibodies	>2 publications with DEFA1 and Liver
Bone Marrow Antibodies	>1 publications with DEFA1 and Bone Marrow