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Egr-2b (egr2b) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643174

Early growth response 2b (egr2b) is also known as krox20. First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Egr-2b (egr2b), Polyclonal Antibody
Full Product Name
Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080)
Product Gene Name
anti-egr2b antibody
Product Synonym Names
Anti -Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes zebrafish Egr-2b.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
45,153 Da
Chromosome Location
Chromosome: 12; NC_007123.5 (9238036..9258480, complement). Location: chromosome: 12
3D Structure
ModBase 3D Structure for Q05159
Immunogen
Synthetic peptide corresponding to the intermediate region of zebrafish Egr-2b.

Product Categories/Family for anti-egr2b antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-egr2b antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-egr2b antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000

Western Blot (WB) of anti-egr2b antibody

Western Blot analysis of Egr-2b in the zebrafish embryonic (24hpf) (A) and larva (6 dpf) (B) lysates with the MBS643174. An immunoreactive band at ~48kD was detected; the band was blocked by the immunizing peptide (C, D).
Rabbit Anti-egr2b Polyclonal Antibody – Cat# MBS643174

Other Notes

Small volumes of anti-egr2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for egr2b purchase

MBS643174 is an antibody targeting egr2b. Also known as Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for egr2b. It may not necessarily be applicable to this product.

NCBI GI #
52138558
NCBI GeneID
30190
NCBI Accession #
NP_571072.2
UniProt Primary Accession #
Q05159
UniProt Related Accession #
Q05159
GenBank Nucleotide #
NM_130997.2
NCBI Official Full Name
early growth response protein 2b
NCBI Official Synonym Full Names
early growth response 2b
NCBI Official Symbol
egr2b
NCBI Official Synonym Symbols
egr2; cb427; krx20; KRX-20; krox20; zgc:92210; id:ibd5073; wu:fb71h10
UniProt Protein Name
Early growth response protein 2b
UniProt Synonym Protein Names
Protein krx-20; Zinc finger protein Krox-20
Protein Family
Early growth response protein
UniProt Gene Name
egr2b
UniProt Synonym Gene Names
egr2; krx-20; krx20; EGR-2b
UniProt Entry Name
EGR2B_DANRE
NCBI Protein Information
early growth response protein 2b; EGR-2b; krox-20; protein krx-20; zinc finger protein Krox-20

UniProt Comments for egr2b

Function: Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.

Subcellular location: Nucleus.

Developmental stage: First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post-fertilization (hpf). By 12 hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14 hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16 hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26 hpf and later in R5 around 30 hpf.

Sequence similarities: Belongs to the EGR C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.
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Research Articles on egr2b

1. Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-egr2b antibody

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 Canonical Wnt - Zebrafish Pathway antibodies  Canonical Wnt - Zebrafish Pathway Diagram
 Neural Crest Development Pathway antibodies  Neural Crest Development Pathway Diagram
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