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POLR2J Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644045

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POLR2J, Monoclonal Antibody
Full Product Name
POLR2J (POLR2J1, DNA-directed RNA Polymerase II Subunit RPB11-a, DNA-directed RNA Polymerase II Subunit J-1, RNA Polymerase II 13.3kD Subunit, MGC71910)
Product Gene Name
anti-POLR2J antibody
Product Synonym Names
Anti -POLR2J (POLR2J1, DNA-directed RNA Polymerase II Subunit RPB11-a, DNA-directed RNA Polymerase II Subunit J-1, RNA Polymerase II 13.3kD Subunit, MGC71910)
Immunogen Sequence
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIE
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1A10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human POLR2J.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
13,293 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (102113547..102119446, complement). Location: 7q22.1
OMIM
604150
3D Structure
ModBase 3D Structure for P52435
Immunogen
Full length recombinant corresponding to aa1-118 from human POLR2J (AAH24165) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-POLR2J antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-POLR2J antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-POLR2J antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-POLR2J antibody

Western Blot detection against Immunogen (38.61kD).
Mouse Anti-POLR2J Monoclonal Antibody – Cat# MBS644045

Western Blot (WB) of anti-POLR2J antibody

Western Blot analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody.|Lane 1: POLR2J transfected lysate (13.3kD).|Lane 2: Non-transfected lysate.
Mouse Anti-POLR2J Monoclonal Antibody, Western Blot (WB) – Cat# MBS644045

Testing Data of anti-POLR2J antibody

Detection limit for recombinant GST tagged POLR2J is 0.3ng/ml as a capture antibody.
Mouse Anti-POLR2J Monoclonal Antibody, Testing Data – Cat# MBS644045

Other Notes

Small volumes of anti-POLR2J antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2J purchase

MBS644045 is an antibody targeting POLR2J. Also known as POLR2J (POLR2J1, DNA-directed RNA Polymerase II Subunit RPB11-a, DNA-directed RNA Polymerase II Subunit J-1, RNA Polymerase II 13.3kD Subunit, MGC71910). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2J. It may not necessarily be applicable to this product.

NCBI GI #
146327795
NCBI GeneID
5439
NCBI Accession #
AAI41831.1
UniProt Primary Accession #
P52435
UniProt Secondary Accession #
O43375; A5D6V8
UniProt Related Accession #
P52435
NCBI Official Full Name
POLR2J protein
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
NCBI Official Symbol
POLR2J
NCBI Official Synonym Symbols
RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB11-a
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit
Protein Family
DNA-directed RNA polymerase
UniProt Gene Name
POLR2J
UniProt Synonym Gene Names
POLR2J1; RNA polymerase II subunit B11-a; RPB11a
UniProt Entry Name
RPB11_HUMAN
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit B11-a; RNA polymerase II 13.3 kDa subunit; DNA-directed RNA polymerase II subunit J-1

UniProt Comments for POLR2J

POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Protein type: RNA splicing; RNA processing; DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; DNA-directed RNA polymerase activity; LRR domain binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; nucleotide-excision repair; positive regulation of viral transcription; somatic stem cell maintenance; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair
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Research Articles on POLR2J

1. POLR2J interacts with three different subunits of eIF3, eIF3a, eIF3i, and eIF3m.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLR2J antibody

 Reference Product  PubMed Publications
 POLR2F antibody  >1 publications with POLR2J and POLR2F
 POLR2C antibody  >1 publications with POLR2J and POLR2C

Pathways associated with anti-POLR2J antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dual Incision Reaction In TC-NER Pathway antibodies  Dual Incision Reaction In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram

Diseases associated with anti-POLR2J antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with POLR2J and Inflammation

Organs/Tissues associated with anti-POLR2J antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with POLR2J and Brain
 Blood Antibodies  >1 publications with POLR2J and Blood
 Lung Antibodies  >1 publications with POLR2J and Lung
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