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MKRN2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644182

The basic function of MKRN2 is mediating ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Makorin RING zinc finger-2 (MKRN2), overlaps and is antisense to the gene RAF1 in mammals. Northern blot analyses show that human MKRN2 and RAF1 are… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MKRN2, Monoclonal Antibody
Full Product Name
MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070)
Product Synonym Names
Anti -MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070)
Immunogen Sequence
AFKQGMGKKA CKYFEQGKGT CPFGSKCLYR HAYPDGRLAE PEKPRKQLSS QGTVRFFNSV RLWDFIENRE SRHVPNNEDV DMTELGDLFM HLSGVESSE*
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5F8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MKRN2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa317-416 from MKRN2 (NP_054879) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-MKRN2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-MKRN2 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-MKRN2 antibody

Western Blot detection against Immunogen (37kD).
Mouse Anti-MKRN2 Monoclonal Antibody – Cat# MBS644182

Western Blot (WB) of anti-MKRN2 antibody

Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody.|Lane 1: MKRN2 transfected lysate (46.9kD).|Lane 2: Non-transfected lysate.
Mouse Anti-MKRN2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644182

Immunofluorescence (IF) of anti-MKRN2 antibody

Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-MKRN2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS644182

Testing Data of anti-MKRN2 antibody

Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.
Mouse Anti-MKRN2 Monoclonal Antibody, Testing Data – Cat# MBS644182

Western Blot (WB) of anti-MKRN2 antibody

Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-MKRN2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644182

Western Blot (WB) of anti-MKRN2 antibody

MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.
Mouse Anti-MKRN2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644182

Other Notes

Small volumes of anti-MKRN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MKRN2 purchase

MBS644182 is an antibody targeting MKRN2. Also known as MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MKRN2. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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