PRDM14 Antibody – Rabbit Polyclonal

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]

PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Protein type: Methyltransferase, protein arginine, predicted; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: nucleoplasm

Molecular Function: methyltransferase activity; protein binding; DNA binding; metal ion binding

Biological Process: fertilization; regulation of transcription, DNA-dependent; transcription, DNA-dependent; somatic stem cell maintenance; homeostasis of number of cells within a tissue; negative regulation of fibroblast growth factor receptor signaling pathway; cell morphogenesis; inner cell mass cell fate commitment; cell fate specification; germ-line stem cell maintenance; regulation of gene expression, epigenetic; germ cell development