Rabbit Anti-DARS2 Polyclonal Antibody – Cat# MBS644519

Western Blot analysis of DARS2 expression in transfected 293T cell line by DARS2 polyclonal antibody. Lane 1: DARS2 transfected lysate (73.6kD). Lane 2: Non-transfected lysate.

DARS2 Antibody – Rabbit Polyclonal

Name: DARS2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS644519
Price: 970 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644519

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. Defects in DARS2 are a cause of leukoencephalopathy with brainstem… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DARS2, Polyclonal Antibody

Full Product Name

DARS2 (Aspartyl-tRNA Synthetase, Mitochondrial, Aspartate-tRNA Ligase, AspRS)

Product Gene Name

anti-DARS2 antibody

Product Synonym Names

Anti -DARS2 (Aspartyl-tRNA Synthetase, Mitochondrial, Aspartate-tRNA Ligase, AspRS)

Immunogen Sequence

MYFPSWLSQL YRGLSRPIRR TTQPIWGSLY RSLLQSSQRR IPEFSSFVVR TNTCGELRSS HLGQEVTLCG WIQYRRQNTF LVLRDFDGLV QVIIPQDESA ASVKKILCEA PVESVVQVSG TVISRPAGQE NPKMPTGEIE IKVKTAELLN ACKKLPFEIK NFVKKTEALR LQYRYLDLRS FQMQYNLRLR SQMVMKMREY LCNLHGFVDI ETPTLFKRTP GGAKEFLVPS REPGKFYSLP QSPQQFKQLL MVGGLDRYFQ VARCYRDEGS RPDRQPEFTQ IDIEMSFVDQ TGIQSLIEGL LQYSWPNDKD PVVVPFPTMT FAEVLATYGT DKPDTRFGMK IIDISDVFRN TEIGFLQDAL SKPHGTVKAI CIPEGAKYLK RKDIESIRNF AADHFNQEIL PVFLNANRNW NSPVANFIME SQRLELIRLM ETQEEDVVLL TAGEHNKACS LLGKLRLECA DLLETRGVVL RDPTLFSFLW VVDFPLFLPK EENPRELESA HHPFTAPHPS DIHLLYTEPK KARSQHYDLV LNGNEIGGGS IRIHNAELQR YILATLLKED VKMLSHLLQA LDYGAPPHGG IALGLDRLIC LVTGSPSIRD VIAFPKSFRG HDLMSNTPDS VPPEELKPYH IRVSKPTDSK AERAH

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human DARS2.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

73,563 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (173793719..173827682). Location: 1q25.1

OMIM

610956

3D Structure

ModBase 3D Structure for Q6PI48

Immunogen

Full length human DARS2, aa1-645 (NP_060592.2).

Product Categories/Family for anti-DARS2 antibody

Antibodies; Abs to Enzymes; Synthase

Applications Tested/Suitable for anti-DARS2 antibody

Western Blot (WB)

Application Notes for anti-DARS2 antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-DARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DARS2 purchase

MBS644519 is an antibody targeting DARS2. Also known as DARS2 (Aspartyl-tRNA Synthetase, Mitochondrial, Aspartate-tRNA Ligase, AspRS). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DARS2. It may not necessarily be applicable to this product.

NCBI GI #

34783643

NCBI GeneID

55157

NCBI Accession #

AAH45173.1

UniProt Primary Accession #

Q6PI48

UniProt Related Accession #

Q6PI48

NCBI Official Full Name

DARS2 protein

NCBI Official Synonym Full Names

aspartyl-tRNA synthetase 2, mitochondrial

NCBI Official Symbol

DARS2

NCBI Official Synonym Symbols

LBSL; ASPRS; MT-ASPRS; RP3-383J4.2

UniProt Protein Name

Aspartate--tRNA ligase, mitochondrial

UniProt Synonym Protein Names

Aspartyl-tRNA synthetase

UniProt Gene Name

DARS2

UniProt Synonym Gene Names

AspRS

UniProt Entry Name

SYDM_HUMAN

NCBI Protein Information

aspartate--tRNA ligase, mitochondrial; aspartate tRNA ligase 2, mitochondrial; aspartyl-tRNA synthetase, mitochondrial

NCBI Summary for DARS2

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]

UniProt Comments for DARS2

DARS2: Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). LBSL is an autosomal recessive disease and is defined on the basis of a highly characteristic constellation of abnormalities observed by magnetic resonance imaging and spectroscopy. Affected individuals develop slowly progressive cerebellar ataxia, spasticity, and dorsal column dysfunction, sometimes with a mild cognitive deficit or decline. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.12; Translation; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; nucleus

Molecular Function: protein binding; protein homodimerization activity; aspartate-tRNA ligase activity; aspartate-tRNA(Asn) ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; gene expression; tRNA aminoacylation

Disease: Leukoencephalopathy With Brainstem And Spinal Cord Involvement And Lactate Elevation

Research Articles on DARS2

1. A comparison of biophysical properties of human mitochondrial aspartyl-tRNA synthetase, HsaDRS2, with them to those of a bacterial (E. coli) homolog, EcoDRS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DARS2 antibody

Products by Pathway	Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Mitochondrial TRNA Aminoacylation Pathway antibodies	Mitochondrial TRNA Aminoacylation Pathway Diagram
TRNA Aminoacylation Pathway antibodies	TRNA Aminoacylation Pathway Diagram

Diseases associated with anti-DARS2 antibody

Disease Name	Pubmed Publications
Leukoencephalopathy with Brainstem and Spinal Cord Involvement and Lactate Elevation Antibodies	>2 publications with DARS2 and Leukoencephalopathy with Brainstem and Spinal Cord Involvement and Lactate Elevation

Organs/Tissues associated with anti-DARS2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>24 publications with DARS2 and Brain
Muscle Antibodies	>3 publications with DARS2 and Muscle
Nerve Antibodies	>2 publications with DARS2 and Nerve
Bladder Antibodies	>1 publications with DARS2 and Bladder